Primary cultures of midbrain neurons were obtained from 15-day-old rat fetuses. Neuron cultures were exposed to ethanol (27 mM, 43 mM and 120 mM) for 24 h and evaluated by light microscopy, a viability measure, and protein content. Ethanol concentrations of 43 and 120 mM appeared to affec...
Further more, primary midbrain neuronal culture was employed to explore the molecular mechanisms leading to axonal degeneration.Our study of WDR45 cKO mice revealed a range of deficits, including impaired motor function, emotional instability, and memory loss, coinciding with the profound reduction of...
Primary rat mesencephalic neuron-glia, neuron-enriched, microglia-enriched, and astroglia-enriched cultures. Methods Mol Med 2003; 79: 387–395. PubMed Google Scholar Wu XF, Block ML, Zhang W, Qin L, Wilson B, Zhang WQ et al. The role of microglia in paraquat-induced dopaminergic ...
This increase in calcium is correlated with a downregulation of the neuron-specific plasma membrane Ca2+-ATPase isoform 2 (PMCA2). Interestingly, two other important mediators of calcium efflux, sarcoplasmic reticulum Ca2+-ATPase (SERCA), and Na+–Ca2+-exchanger (NCX), remained unaltered, ...
Microglia play a central role in neuroinflammation and may affect neuron vulnerability. Next generation sequencing has shown the molecular heterogeneity of microglial cells; however, the variability in their response to pathological inputs remains unknown. Methods To determine the effect of an inflammatory...
Raab, Nongenomic effects of oestrogen: embryonic mouse midbrain neurones respond with a rapid release of calcium from intracellular stores, Eur. J. Neurosci. 10 (1998) 255 - 262.Beyer, C. & Raab, H. (1998) Nongenomic effects of oestrogen: embryonic mouse midbrain neurones respond with a...
in triplicate for 2-to-3 distinct primary culture batches. Standard error of the mean values were graphed, analyzed by student’st-test, andp < 0.05 were considered significant. Live-cell lysosomal GCase activity assay. The procedure and analysis method for the activity assay has been ...
The invention provides a method for directionally differentiating neural stem cells in human embryo midbrain into dopaminergic neuron in vitro, and the method comprises the following steps of: isolated culture of primary cells; continuous cell culture of neural stem cells; induced differentiation culture...
d DQ BSA proteolysis live imaging in mixed genetic neuron/astrocytes co-cultures (n = 12, Two-way ANOVA followed by Tukey’s for the multiple comparisons test). e Western blot for GFAP and actin (ACTB) loading control for Neurons/Astrocytes co-cultures and quantification (n = 7,...
The midbrain dopamine (mDA) neuron is of particular interest because of its significant physiological and clinical implications. Parkinson's disease (PD), characterized by progressive loss of mDA neurons in the midbrain substantia nigra (SN),1,2 is the only neurodegenerative disease with a ...