primary cellscell linesimmortalizationin vitro cell culturestandardizationdoi:10.1038/npg.els.0002564StaceyGlynStacey, G. (2001). Primary Cell Cultures and Immortal Cell Lines. DOI: 10.1038/npg.els.0003960.Stacey G, Bar P, Granville R (2009) Primary cell cultures and immortal cell lines. Encycl ...
2.1.1.4 Cell Culture Scientists can culture cells taken from original tissue, primary cultures, cell lines, or cell strains. Late embryonic rat hippocampus and human tumor specimens are examples of original tissues. Numerous continuous cell lines are widely available to researchers. Each continuous cel...
The recent development of the nucleofection system has been a significant advance over standard electroporation systems that have been limited by high toxicity and a requirement for large numbers of cells. A number of cell lines have already been tested for their compatibility with the nucleofection ...
Infection of human primary B-lymphocytes with Epstein-Barr virus (EBV) drives quiescent cells into continual proliferation and results in the outgrowth of immortal cell lines. This requires re-programming of the mechanisms that, in the absence of appropriate antigenic stimulation, normally prevent the...
In addition, we suggest that in crayfish, the hematopoietic system may be a source of cells that replenish the niche stem cell pool.Background It is now well established that neurogenesis occurs not only during embryogenesis and early postembryonic development, but also in the brains of many ...
“driver”mutations contributing to the development of the cancers in 119 genes. Out of 210 cancers analyzed, 169 were primary tumors, 2 were early cultures, and 39 were immortal cancer cell lines. Pre 多数体壁变化预计是“对oncogenesis不贡献的乘客”,但是证据为“贡献对癌症的发展的司机”变化在...
Alpha TC1 (αTC1) and Beta-TC-6 (βTC6) mouse islet cell lines are cellular models of islet (dys)function and type 2 diabetes (T2D). However, genomic characteristics of these cells, and their similarities to primary islet alpha and beta cells, are undefined. Here, we report the epigeno...
Cellular prion protein suppressess the apoptotic cell death by mediating the intracellular H 2 O 2 in primary culture and immortalized neuronal cellsTo know the mechanisms in which cellular isoform of prion protein (PrPC) is involved for the neuroprotection, we compared death signals in Prnp-/- ...
cells derived from a finite population of cells (~5-6 passages) (22). Only two specimens of resected colorectal tumor were used to establish cell lines; one cell line was cc-006cpm8 and was established in the present study for cellular characterization. In addition, the patient was ...
Keywords: primary cells; cell lines; immortalization; in vitro cell culture; standardizationdoi:10.1038/npg.els.0003960Glyn StaceyJohn Wiley & Sons, LtdStacey, G. (2001). Primary Cell Cultures and Immortal Cell Lines. DOI: 10.1038/npg.els.0003960....