Usually the primary cells are obtained to establish a culture that is composed of a single cell type. In some cases, however, the goal is to establish a mixture of cell types in the culture system. Examples are organ culture and mixtures of cells to examine cell interaction (see Laerum ...
In this work we ask how primary cells and cell lines differ in their functions. This question has been addressed by comparing gene expression profiles at the transcriptome level in a substantial body of literature (for recent examples, see Refs. 7, 8). However, transcriptome studies are not ...
IS formation is the first step towards T-cell activation and essential for clonal expansion and acquisition of effector function. We here evaluated PIDs in which aberrant f-actin-driven IS formation may contribute to the PID disease phenotypes as seen in patients. We review examples of such ...
Examples of existing techniques and solutions are: It is inefficient to use centralized solutions to manage large networks because they usually impose high overheads. Using clustered IoT networks, the authors often present a scalable MQTT model. Message brokers serve as part of each cluster. MQTT ...
passaged cell lines is adapted to their highly proliferative state rather than being representative of the tissues from which they were derived [56,57]. Therefore, we sought to establish a method that allowed us to query the function of regulatory elements in a physiologically relevant cell ...
Considering the examples provided in our work, we believe that the Nanoblade technology will facilitate gene editing in academic laboratories working with primary cells and could represent a viable alternative for therapeutical purposes and the rapid generation of primary cell-types harboring genetic dise...
Finally, our three examples of clonal evolution highlight DNA clonality shifts coincident with gene expression-based subtype changes. In participant AER8, the clonal shift and altered expression subtype did not include any new actionable mutations, which may represent the most common finding with res...
Case examples Case 12The proband was a 24-year-old male who presented with for “paroxysmal limb weakness for 6 years”. The patient developed weakness in the limbs after having a cold and fever 6 years ago. Electrolyte examination at the local hospital indicated hypokalemia. After intravenous ...
Illustrative examples of images taken at mid-height of the cells are shown in Fig. 5b. Spike/vimentin colocalization was quantified using Manders’ coefficients as the percentage of Spike overlapping with the vimentin immunoreactive signal (Fig. 5b graph). Significant colocalization of Spike S1 and...
Other objects and features of the invention will become apparent from the following description and accompanying drawings given only as examples wherein: FIG. 1 is a front elevation of a collector means according to the present invention;