Each primary antibody was diluted 1:200 in blocking solution. The slide-mounted samples were incubated with the primary antibodies overnight at 4 °C, after which they were washed three times for 10 min with PBS + 0.1% (v/v) Triton X-100. The slides were incubated with the ...
The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest. Volume (to add to vial) ...
analyzed using Mann-Whitney statistical test. ****p < 0.0001.EDot plot quantification of AQP5 in two-fold serial dilutions of ED protein extracts from Ctl and cKO mice. To control for specificity, the antibody was pre-incubated with a 10-fold excess of the AQP5 immune peptide.E’AQP...
USA). Primary antibodies used were rabbit monoclonal anti-alpha tubulin antibody (dilution 1:1000; ab179484, Abcam, Cambridge, UK), Rabbit Polyclonal Anti-inversin antibody (dilution 1:100; ab65187, Abcam, Cambridge, UK), mouse monoclonal DVL-1 antibody (1:150 dilution; sc-8025, Santa Cruz...
Cited in 2 publications. View Human CD23/Fc epsilon RII Antibody (MAB1231) validated in Human. Additional non-catalog clones available.
dilution experiments in WT mice, Thy1.2 enrichment was performed using anti-Thy1.2 antibody and anti-APC magnetic Microbeads (Miltenyi Biotec) following manufacturer protocol. For Nur77 analysis in WT mice flow-through (FT) samples were used and not enriched. For all cases, prepared samples were...
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas Human/Mouse RASSF2 Antibody Summary Species Reactivity Human, Mouse Specificity Detects endogenous human and mouse RASSF2 in Western blots. Source Poly...
The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest. Volume (to add to vial) ...
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas Human Cytokeratin 19 Biotinylated Antibody Summary Species Reactivity Human Specificity Cytokeratin 19 antibodies are ideal for immu...
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas Human/Mouse/Rat SEC13 Antibody Summary Species Reactivity Human, Mouse, Rat Specificity Detects human SEC13 in direct ELISAs and W...