免疫染色一抗稀释液(Immunol Staining Primary Antibody Dilution Buffer)可以用于免疫染色时一抗(primary antibody)的稀释和配制。使用免疫染色一抗稀释液稀释和配制的一抗可以在4℃保存和使用6个月,可以反复多次用于免疫染色。按照每个一抗稀释10ml计算,100ml免疫染色一抗稀释液可以稀释10次一抗。
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Conjugate SourceN/A Size60ml ApplicationImmunology StorageStore at 2-8℃ for six months. SynonymsWestern Antibody Diluent;Antibody Diluent;Primary Antibody Dilution Buffer for Western Blot;Western Primary Antibody Dilution Buffer;Antibody Diluent(TBST);Western一抗稀释液;Western一抗稀释缓冲液;Western抗体稀释...
产品简介:—抗稀释液(Primary Antibody Dilution Buffer)可以用于 Western Blot时一抗(Primary Antibody)的稀释和配制。使用本—抗稀释液稀释和配制的一抗可以在4℃保存和使用不少于1个月,可以反复多次用于Western Blot,节约抗体。按照每个一抗稀释10mL计算,一个包装的一抗稀释液可以稀释10次或50次一抗。
Primary Antibody Dilution Buffer for Western Blot|WESTERN BLOT一抗稀释液 基本信息更多信息 中文名称:WESTERN BLOT一抗稀释液 中文同义词:WESTERN BLOT一抗稀释液 英文名称:Primary Antibody Dilution Buffer for Western Blot 英文同义词:Primary Antibody Dilution Buffer for Western Blot ...
After being soaked with 5% non-fat milk (0.05% Tween, 20% TBST) for 1.5 h at room temperature to block the non-specific binding, the membrane was incubated with 1:1000 dilution of the primary antibody in dilution buffer (Abs954, Absin, China) overnight at 4 °C. Antibodies specific ...
developing a complete IHC workflow using primary antibodies raised in mice.The kit contains all the basic reagents,starting from antigen retrieval buffer to mounting media,that would be required for IHC staining of tissue sections with mouse primary antibodies.By simply optimizing the primary antibody ...
000 cells and 10 μL of beads were used. The unbound supernatant was removed, and bead-bound cells were resuspended in 50 μL ice-cold Antibody buffer (20 mM HEPES pH 7.5, 150 mM NaCl, 0.5 mM Spermidine, 10 mM Sodium butyrate, 0.4 mM EDTA, 0.02% BSA, 0.05% Digitonin, 1X Protease...
000 cells and 10 μL of beads were used. The unbound supernatant was removed, and bead-bound cells were resuspended in 50 μL ice-cold Antibody buffer (20 mM HEPES pH 7.5, 150 mM NaCl, 0.5 mM Spermidine, 10 mM Sodium butyrate, 0.4 mM EDTA, 0.02% BSA, 0.05% Digitonin, 1X Protease...
Reduce detergent concentration. Typical detergents and concentrations (in primary antibody incubation buffer) include either Triton X-100 (0.05–1%) or Tween 20 (0.05–2%). This technique is especially applicable to aldehyde-based fixation methods. ...