coverage for miRBase v21 - Format: Individual tubes, TaqMan array cards and plates, and OpenArray plates TaqMan Advanced miRNA Assay: - RT Chemistry: Employs a universal RT primer during cDNA synthesis - Pr...
TaqMan Pri-miRNA Assays Detect the origins of microRNA expression • Highly specific—quantitate microRNA transcription from a single genomic locus • Fast, simple, and scalable—two-step RT-qPCR provides high-quality results • A new perspective—measure microRNA expression at the gene level ...
进一步通过miRNAs测序发现SOP1仅影响一小部分miRNAs,例如一些与发育相关的miRNAs (如miR156、miR159、miR162和miR319)。作者也通过Northern blot分析了miR159、miR160和miR319的水平,发现与hyl1-2相比,在hyl1-2 sop1-6中部分回复恢复。qPCR分析显示,miR156、miR159、miR160和miR319的靶基因在hyl1-2中升高,在hyl...
RT-qPCR was applied to determine mRNA of m6A-associated genes and SFRP2,... S Zhao,P Song,DHY Zhou - 《Cancer Gene Therapy》 被引量: 0发表: 2023年 METTL3‐mediated m6A modification of pri‐miR‐148a‐3p affects prostate cancer progression by regulating TXNIP Objective: Prostate cancer (...
i, RT–qPCR analysis of pri-miRNAs in single and double mutants relative to Col-0. Total RNA was prepared from a pool of 21-day-old plants; EF-1α serves as a loading control. The relative signals of pri-miRNAs were normalized to those of wild-type Col-0, where the ratio was ...
Previous studies have shown that peptides encoded by noncoding RNAs (ncRNAs) can be used as peptide drugs to alleviate diseases. We found that microRNA-31 (miR-31) is involved in the regulation of hypertension and that the peptide miPEP31, which is encod
miR-221-3p inhibition resistance and METTL3 overexpression was performed in MCF-7/ADR cells by using a miR-221-3p-specific inhibitor and expression vector containing METTL3, which was demonstrated by RT-qPCR and immunoblotting (Fig. 3a, b). We then performed MTT assays and Annexin V-FITC/...
miR-221-3p inhibition resistance and METTL3 overexpression was performed in MCF-7/ADR cells by using a miR-221-3p-specific inhibitor and expression vector containing METTL3, which was demonstrated by RT-qPCR and immunoblotting (Fig. 3a, b). We then performed MTT assays and Annexin V-FITC/...
We examined the expression of methyltransferase-like 3 (METTL3) in tumor specimens using RT-qPCR, immunohistochemistry, and Western blot analysis, and tested the methylation of METTL3 by MSP. Levels of METTL3, miR-1246, pri-miR-1246 and CCNG2 were then analyzed and their effects on cell ...
然而,由于m6A在调控生长素应答中的重要作用,作者进一步研究了m6A对miR393b 累积的需求。作者使用m6A-IP和RT-qPCR确认了pri-miR393b是m6a -甲基化。与MTA结合的IP RNA也证实了MTA与pri-miR393b的结合(图6B)。值得注意的是,作者的PIP-seq分析显示,pri-miR393b在mta中的结构比WT中的要少。