Examination of mRNA expression in these cells revealed that c-Myb controls expression of key regulators of the pre-BCR checkpoint, including CD127, lambda-5, cyclin D3 and Cxcr4. c-Myb coordinates survival in pro-B cells with the expression of key components of the signaling pathways that ...
(2001) Negative selection at the pre-BCR checkpoint elicited by human μ heavy chains with unusual CDR3 regions. Immunity 14, 631-641 doi:10.1016/S1074-7613(01)00131-5Minegishi Y, Conley ME. Negative selection at the pre-BCR checkpoint elicited by human mu heavy chains with unusual CDR3 ...
EarlyIgkrearrangement enables B cells to bypass the pre-BCR checkpoint.aIgkrecombination can occur independent ofIghrecombination as shown by semiquantitative PCR performed on ex vivo-derived fetal liver pro-B cells E17.5 (Lin−CD19+B220+IgM−CD2−CD117+) from wild-type and JHT mice. Eac...
Although only transiently expressed, the pre-BCR marks an important checkpoint in B-cell development and regulates diverse processes, such as proliferation, allelic exclusion of the second heavy chain allele, termination of SLC expression and initiation of immunoglobulin light chain (IgL) gene ...
Restoration of BLNK expression in Igμ+ Pax5−/− pro-B cells rescued the proliferative function of the pre-BCR and facilitated a systematic analysis of the gene expression changes initiated by pre-BCR signaling. These microarray data demonstrated that the pre-BCR executes its checkpoint ...
During B cell development, the precursor B cell receptor (pre-BCR) checkpoint is thought to increase immunoglobulin κ light chain (Igκ) locus accessibility to the V(D)J recombinase. Accordingly, pre-B cells lacking the pre-BCR signaling molecules Btk or Slp65 showed reduced germline Vκ tra...
During B cell development, the precursor B cell receptor (pre-BCR) checkpoint is thought to increase immunoglobulin κ light chain (Igκ) locus accessibility to the V(D)J recombinase. Accordingly, pre-B cells lacking the pre-BCR signaling molecules Btk or Slp65 showed reduced germline Vκ tra...
The pre-B celt receptor (pre-BCR) serves as a checkpoint in B cell development. In the 2.7 angstrom structure of a human pre-BCR Fab-like fragment, consist... AJ Bankovich,S Raunser,ZS Juo,... - 《Science》 被引量: 252发表: 2007年 Adaptation to Climate Change in the Construction ...
To test whether pre-BCR-induced downregulation of EFhd1 serves as a metabolic checkpoint, we generated EFhd1 transgenic mice (EFhd1tg) expressing EFhd1 under control of the Eμenhancer and VHpromoter (Figure 3f). Southern blot and circle PCR analysis revealed that seven copies of the transgene...
among the genes involved in this process are those regulating the mitotic checkpoint, DNA damage response, and recombination, as might be the case in the pre-B ALL patients studied here. Likewise, genomic alterations may disrupt many pathways at once; for instance, Simonetti et al. [27] obser...