Here, we highlight recent insights into the contribution of the PRC2 facultative subunits to PRC2 localization at target genes and the regulation of the catalytic activity of PRC2 and discuss how PRC2 subcomplexes might cooperate to regulate PRC2 function. We mainly focus on PRC2 in mouse emb...
Short RNAs are transcribed from repressed polycomb target genes and interact with polycomb repressive complex-2 Mol. Cell, 38 (2010), pp. 675-688 Google Scholar Khalil et al., 2009 A.M. Khalil, M. Guttman, M. Huarte, M. Garber, A. Raj, D. Rivea Morales, K. Thomas, A. Presser...
Together, PRC1 and PRC2 silence critical target genes involved in fundamental cellular processes such as cell fate decision, cell differentiation, cell cycle regulation, senes- cence and cancer[1, 6, 7, 11, 12]. Dysregulation of PRC2 is involved in a wide variety of can- cers, making the...
The PRC2 complex is enriched on the Xi and, similar to ATRX, interacts with Xist lncRNA20,21,22. Interestingly, while ATRX itself is not enriched across the Xi, depletion of ATRX results in the loss of PRC2 from genes on the Xi as well as from other polycomb target genes on ...
This finding suggests that changes to proximal amino acids in the H3 N-terminal tail alter distinct biological pathways. We exploited the caffeine sensitive growth of H3K36 mutant cells to perform a high copy suppressor screen. This screen identified genes linked to histone function and ...
results in growth inhibition due to cell cycle arrest and apoptosis. Resistance to EZH2 inhibitor is mediated by DNA methylation of PRC2 target genes. We also observed a synergy between EPZ-6438 and lenalidomide, a conventional drug used for MM treatment. More interestingly, pretreatment of myeloma...
In this work, we explored the ALKBH1 genome-wide targets and its function in 6mA demethylation, chromatin modifications, and gene expression in plants. Our study uncovered that the ALKBH1-binding antagonizes PRC2 function to maintain a high H3K4me3/H3K27me3 ratio in its target genes to upregu...
Here, we show a profound and cancer-selective role for PRC2 in repressing multiple cytokine pathways. We find that PRC2 represses hundreds of IFN纬 stimulated genes (ISGs), cytokines and cytokine receptors. This target repertoire is significantly broadened in cancer vs non-cancer cells, and is ...
TARGETCELLSPolycomb group (PcG) proteins maintain transcriptional repression of developmentally important genes(1) and have been implicated in cell proliferation and stem cell self-renewal2. We used a genome-wide approach(3) to map binding patterns of PcG proteins (Pc, esc and Sce) in Drosophila...
While tremendous progress has been made into understanding specific genes and pathways that regulate HSC self-renewal, the molecular mechanisms by which self-renewal is repressed in downstream hematopoietic progenitors is largely unknown. Understanding the mechanisms that restrict self-renewal potential in ...