Twenty-four hours post-transfection, both control and PP6c knockdown cells were transfected with the FLAG-R1 plasmid. Forty-eight hours post-transfection, FLAG-R1 was immunoprecipitated (IP) from the lysates of these cells using anti-FLAG antibody. The levels of input proteins (top three panels...
parental (WT) and olaparib-resistant (olaR); PEO1 empty vector and myc-tagged CCDC6 overexpressing cells; PEO4 silenced for CCDC6 (shCCDC6) or control (shCTRL), as in plasmid and transfection paragraph.
The reporter plasmid DR-GFP consists of two mutated GFP cassettes. The GFP cassette at the top contains a binding site for I-SceI restriction enzyme, whose expression causes a Double Strand Break (DSB). In Homologous Recombination (HR) proficient cells, the GFP available in the cassette on ...
The flanking regions were amplified from WT H99 genomic DNA and NEOR from plasmid pJAF1 (a gift from Dr John R Perfect, Duke University, Durham, NC, USA)39. The deletion construct, which effectively had NEOR in place of the IPK1 coding region, was then transformed into the WT H99 ...
DNA sequence determination and functional characterization of the OCT-plasmid-encoded alkJKL genes of Pseudomonas oleovorans - Beilen, Eggink, et al. - 1992 () Citation Context ...s oleovorans is able to grow on alkanes and alkenes (1). Its catabolic OCT plasmid contains two operons that ...
In Comamonas testosteroni BR60 (formerlyAlcaligenes sp. strain BR60), catabolism of the pollutant 3-chlorobenzoate (3CBA) is initiated by enzymes encoded by cbaABC, an operon found on composite transposon Tn5271 of plasmid pBRC60. ThecbaABC gene product CbaABC converts 3CBA to protocatechuate...
Plasmid pRN1 from Sulfolobus islandicus REN1H1 is believed to replicate by a rolling circle mechanism but its origin and mechanism of replication are not w... CJ Joshua,LD Perez,JD Keasling - 《Plos One》 被引量: 8发表: 2013年 Functional Characterization of the Origin of Replication of pR...