Assay of potential Tre-related off-target effects.Ilona HauberHelga HofmannSieberJan ChemnitzDanilo DubrauJanet ChusainowRolf StuckaPhilip HartjenAxel SchambachPatrick ZieglerKarl Hackmann
Inhibition of genes is a powerful approach to study their function. While RNA interference is a widely used method to achieve this goal, mounting evidence indicates that such an approach is prone to off-target effects. An alternative approach to gene function inhibition is genetic mutation, such ...
IADBtreatment in combination with chemotherapy can mitigate 5–Fluorouracil-Induced Cardiotoxicity. The study about IADB may support novel therapeutic targets to improve chemotherapeutic efficacy with decreased off-target effects. Meng and his partner demonstrated thatfascaplysin, a red pigment isolated from...
were included in downstream analyses to evaluate putative off-target effects. We performed H3K27ac and H3K9me3 ChIP-seq in the same cells to assess the epigenetic changes imparted by CRISPRi. We quantified the ratio in histone modification levels at dCas9 peaks between cells expressing LTR2B s...
Cas-OFFinder: a fast and versatile algorithm that searches for potential off-target sites of Cas9 RNA-guided endonucleases. Bioinformatics. 2014; 30 :1473–5. doi: 10.1093/bioinformatics/btu048. [ Cross Ref ]... D Zhao,J Li,S Li,... - 《Nature Biotechnology》 被引量: 0发表: 0年 Expe...
otheralgorithmscurrentlyavailableforidentificationofRGENoff-targetsites,Cas-OFFinderisnotlimitedbythenumberofmismatchesandallowsvariationsinprotospacer-adjacentmotifsequencesrecognizedbyCas9,theessentialproteincomponentinRGENs.Cas-OFFinderisavailableasacommand-lineprogramoraccessibleviaourwebsite.Availabilityandimplementation:...
The higher-frequency parts of the signal allows in particular capturing the effects of the combinatorial logic that is between the latches of the circuit. The results of EM attacks can be improved by opening the packaging of the device (see below). In analogy to power side-channel attacks, ...
cut off die剪模 bridge cut off桥式断路 targetn. 1. 目标,靶 2. 受批评等的人或事物,批评的目标、 对象 3. 目的,目标 v. [T][~ sth (at/on sth/sb)] 瞄准某物 potentialadj. 潜在的,可能的 n.[U] 1. 可能性,潜在性 2. 潜力,潜能 3.【术语】电势,电位 ...
many immunogenic reports of lentiviral delivered Cas9, however, constantly expressed CRISPR/Cas9 may give rise to undesirable off-target effects. ‘Hit and run’ therefore is a better strategy, such as electroporating ribonucleoprotein (RNP) or plasmids. However, in our initial attempts to electro...
Off-target effects related to the phosphorothioate modification of nucleic acids. ChemMedChem 5, 1344–1352 (2010). Article CAS PubMed Google Scholar Lai, J. C. et al. A pharmacologic target of G3139 in melanoma cells may be the mitochondrial VDAC. Proc. Natl Acad. Sci. USA 103, 7494...