Cyt579-enriched samples were desalted with Zip-Tip (C4, Millipore, Billerica, MA, USA) pipette tips and eluted with 100% ACN (0.1% acetic acid, v/v). The protein fraction was then diluted into 50/50/0.1 (v/v/v) H2O/ACN/acetic acid and infused into the Micromass Z-Spray source ...
The CFTR cooperative domain-folding model dictates that following the formation of secondary and some tertiary structural elements co-translationally, the native domain-swapped conformational ensembles develop posttranslationally15, which requires dynamic and/or energetic interdomain coupling and the expression ...