Methods and Materials Animals, mouse husbandry and handling. All research was conducted according to the National Institute of Health's Office of Laboratory Animal Welfare (OLAW) guidelines as described in Guide for the Care and Use of Laboratory Animals regulations. All ethics were reviewed and...
Cyt579-enriched samples were desalted with Zip-Tip (C4, Millipore, Billerica, MA, USA) pipette tips and eluted with 100% ACN (0.1% acetic acid, v/v). The protein fraction was then diluted into 50/50/0.1 (v/v/v) H2O/ACN/acetic acid and infused into the Micromass Z-Spray source ...
Intramolecular allostery has been established to regulate the CFTR and MRP1 function7,24,27,50,51,60,61,62. The observation that the ΔF508-NBD1 primary folding defect and its global consequences in the core domains were rescued by both suppressor mutations in cis or correctors binding to the...
Osteopontin (OPN) is a phosphorylated glycoprotein secreted into body fluids by various cell types. OPN contains arginine-glycine-aspartate (RGD) and serine-leucine-alanine-tyrosine (SLAY) motifs that bind to several integrins and mediate a wide range of cellular processes. In the present study, t...
The chemical composition was determined using a Bruker WD XRF S8 Tigerfluorescence spectrometer (Bruker Optics Inc, Billerica, Massachusetts, USA). The waste sample was ground in a MM400 Retsch ball mill (Retsch, Hahn, German)in zirconium oxide vessels and then pressed without a binder in an ...
Microwave Assisted Acid Digestion of Sediments, Sludges, Soils, and Oils; Office of Solid Waste and Emergency Response, U.S. Government Printing Office: Washington, DC, USA, 1998. 21. Pueyo, M.; López-Sanchez, J.F.; Rauret, G. Assessment of CaCl2, NaNO3 and NH4NO3 extraction procedures...