Methods and Materials Animals, mouse husbandry and handling. All research was conducted according to the National Institute of Health's Office of Laboratory Animal Welfare (OLAW) guidelines as described in Guide for the Care and Use of Laboratory Animals regulations. All ethics were reviewed and...
Mouse monoclonal anti-CFTR Ab MM13-4 (1:500, specific to the N-terminal a.a. 25-36, #05-581) was purchased from Millipore (Billerica, MA). Anti-human MRP1 monoclonal Abs: QCRL-1 (1:1000, specific for a.a. 918-924, #SC-18835) was purchased from Santa Cruz Biotechnology. MRPr1...
including the brain, and it has been reported that OPN has important roles in various physiological processes, such as anchoring osteoclasts to the mineral bone matrix during bone resorption1and attaching calcium oxalate crystals to renal tubules in stone formation.2Elevation of OPN expression has bee...
After filtration, the digestion products were adjusted to 100 mL volume with Milli-Q water (18.2 MΩ, Milli-Q Element A10 purification system, Millipore, Billerica, MA, USA). Extracts were analyzed for TE concentrations determined by the Atomic Absorption Spectrometry (AAS) method using an iCE...