Cyt579 intact mass measurement Purified samples of Cyt579 were further prepared for characterization of the intact proteins by high- resolution top-down MS. Cyt579-enriched samples were desalted with Zip-Tip (C4, Millipore, Billerica, MA, USA) pipette tips and eluted with 100% ACN (0.1% ...
Here, we employ strategies to investigate the molecular/cellular mechanism of complex domain-folding and -misfolding of ABCC-transporters, as well as the rescue mechanism of the VX-809 and VX-445 CFTR folding correctors that may provide life-changing improvements for CF patients45. Using targeted ...
After filtration, the digestion products were adjusted to 100 mL volume with Milli-Q water (18.2 MΩ, Milli-Q Element A10 purification system, Millipore, Billerica, MA, USA). Extracts were analyzed for TE concentrations determined by the Atomic Absorption Spectrometry (AAS) method using an iCE...