Fig. 1: Mor-Ens drive positive reinforcement in a DA-dependent manner. aExperimental process of ensemble labeling. Mice were intraperitoneally injected with TAM (125 mg/kg), and morphine (10 mg/kg) or saline was injected 24 h later. Immunostaining was carried out four days after the la...
Remarkably, we found that TOP3B is required for efficient replication of all positive-sense-single stranded RNA viruses tested, including SARS-CoV-2. While there are no drugs that specifically inhibit this topoisomerase, we posit that TOP3B is an attractive anti-viral target....
(E and F) TuMV +RNA (E) and CP (F) accumulation in TuMV-GFP-WT- or TuMV-GFP-mrORF-inoculated N. benthamiana plants at 5 dpi as detected by qRT–PCR and western blotting, respectively. (G) Disease symptoms in TuMV-GFP-WT- or TuMV-GFP-mrORF-inoculated N. benthamiana plants at 12...
Two-component SystemPrevious studies constructed a chimeric MalKZ two-component system to sense environmental malate. In this study, we used a positive feedback loop to accelerate and amplify the output signal indicating malate concentration. The positive feedback loop was constructed by cloning ompR ...
Digoxigenin UTP-labelled sense and antisense RNA probe were generated by in vitro transcription of the zero blunt plasmid having the amplicon of interest, post linearisation with ECORV/BamH1 (NEB #R3195L and #R3136L), respectively, and stored at −80 °C for further use in ISH. In ...
coli TOP10 harbouring gfp fusion plasmid and pJV-300 (named as “no sRNA::5′ UTR sipA-gfp”) in both whole-cell colony plate and whole-cell liquid culture assays to determine the interaction between RyhB and sipA. The same method was also used to construct sopE related expression ...
2a, a significantly higher proportion of pseudogene on sense strand than on antisense strand has gained m6A motifs, supporting positive natural selection of the gained m6A motifs on pseudogenes (P = 1.4× 10−35, two-tailed chi-square test). Fig. 2 Positive natural selection of the m6A...
"The Forte 44, the Threshold T2, the Pass Labs Aleph P preamplifier and Aleph P remote, the Adcom GFP 750 preamplifier, the Pass Labs X0 preamplifier, the Pass Labs X1 preamplifier, the Pass Labs X2.2 preamplifier, the Pass Labs X2.5 preamplifier, the Pass Labs X0.2 preamplifier, ...
It is of interest to note that certain biological effects of SAR values in the range of 4.0 kW kg−1 at 8.53 GHz and 4.85 kW kg−1 at 2.45 GHz, have been described, e.g., three-dimensional conformational changes in green fluorescent protein (GFP)25 and increased ...
Current transformers 24 surround each of the internal phase conductors 16 to sense the level of current flow through the conductors 16. The output from the current transformers 24 is supplied to a trip unit 26, along with the output from a current transformer 28 which senses the level of grou...