The introduction of pHluorin, a pH-sensitive GFP, by Miesenbock and colleagues provided a versatile tool to studies of vesicle trafficking, in particular synaptic vesicle exocytosis and endocytosis. By tagging pHluorin to the luminal region of the synaptic vesicular protein synaptobrevin (also ...
The red fluorescence represents the fluorescent protein mCherry, and the green fluorescence represents MyrPalm-GFP. The scale bar is 40 μm. The cytotoxicity of P(C6-Bnx) (c) and P(C6-R20) (d) copolymers (160 μg ml-1) against Panc02 cells at different pH conditions after 2 h ...
and separately inserted into Pgp64-GFPpFB, which generated by replacement of the promoter and ORF of GP64 in GP64pFB52with a fused fragment containing the promoter of AcMNPVgp64and the ORF of GFP, to generate the pFastBac plasmids Pgp64-XpFB and Pgp64-Myc-YpFB. A synthesized fragment of...
INTRODUCTION S65T mutations, changes that markedly enhance flores- The green florescent protein (GFP) from Aequorea vic- cent output at the 475 nm excitation peak while main- toria and its many engineered variants have revolution- taining pH sensitivity. The superecliptic pHluorin is ized the ...
The invention is directed to dual-analyte fluorescent chemosensors for the direct detection and visualization (imaging) of neurotransmitters released upon exocytosis. The inventive sensor exploits the high concentration of neurotransmitters (e.g., glutamate, norepinephrine, and dopamine) and capitalizes upon...
(TGFβ) inhibitors. TGFP inhibitors that can be used include P144, a fourteen amino acid long peptide that inactivates TGFβ and has been shown to reduce soluble collagen content in skin fibrosis 2) SB-525334, a small molecule TGFp inhibitor with a polyaromatic-ring-structure shown to ...
Here the application of pH-sensitive GFP technology in plants is described and, for the first time, the visualization of pH gradients between different developmental compartments in intact whole-root tissues of A. thaliana is reported. The utility of pH-sensitive GFP in revealing rapid, ...
pH-sensitive mutants of green fluorescent protein are also provided, which are useful for visualizing exocytosis and for imaging and measuring the pH of intracellular compartments.Miesenbock GeroDe Angelis Dino A.Rothman James E
A pH-sensitive GFP variant, the so-called pHluorin, has proven to be a valuable tool to study the pH of the cytosol, mitochondria and other organelles in vivo. We found that the fluorescence intensity of Endoplasmic Reticulum (ER)-targeted pHluorin in the yeast Saccharomyces cerevisiae was ...
pH-sensitive mutants of green fluorescent protein are also provided, which are useful for visualizing exocytosis and for imaging and measuring the pH of intracellular compartments.Miesenbock GeroDe Angelis Dino A.Rothman James E