Regulator of G protein signaling (RGS) proteins increases the intrinsic GTPase activity of G伪-subunits and are widely regarded as negative regulators of G protein signaling. Using yeast we demonstrate that GTP hydrolysis is not only required for desensitization, but is essential for achieving a ...
Notably, Git2(-/-) double-positive thymocytes showed greater activation of the small GTPase Rac, actin polymerization and migration toward the chemokines CXCL12 (SDF-1) and CCL25 in vitro. By two-photon laser-scanning microscopy, we found that the scanning activity of Git2(-/-) thymocytes ...
Signal inactivation requires Sst2, a regulator of G protein signaling (RGS) protein that accelerates GTPase activity. We conducted a quantitative analysis of RGS and G protein expression and devised computational models that describe their activity in vivo. These results indicated that pheromone-...
Moreover, we examined whether upregulation of Jagged-1 and PTHrP requires PAK4 kinase activity. MCF-7 and ZR-75-30 cells were treated with PF-3758309, and the results indicated that the regulation of Jagged-1 and PTHrP by PAK4 depended on its kinase activity (Figure 4G). To further ...
This inhibition dysregulates the divisome and elongasome supported by an upregulation of key proteins FtsZ, DivIVA, and MreB inducing cell division arrest. The inhibition of ClpXP and ClpYQ to dysregulate cell division represents a unique antibiotic mechanism of action and armeniaspirol is the ...
the CCR4-NOT complex is up-regulated in thymocytes before initiation of positive selection, where in turn, it inhibits up-regulation of pro-apoptotic Bbc3 and Dab2ip. Elimination of the CCR4-NOT complex permits up-regulation of Bbc3 during a later stage of positive selection, inducing thymocyte...
fusion-positive prostate cancer cells; 4) miRNA mediated regulation of the androgen receptor (AR) and its associated protein network; 5) Quantitative control of ERG in prostate cancer cells; 6) TMPRSS2-ERG encoded protein targeting; In conclusion, we provide a detailed understanding of TMPRSS2-...
Chakrabarty A, Sanchez V, Kuba MG, Rinehart C, Arteaga CL . Feedback upregulation of HER3 (ErbB3) expression and activity attenuates antitumor effect of PI3K inhibitors.Proc Natl Acad Sci USA2011;109: 2718–2723. ArticleGoogle Scholar
Constitutively active Rheb-overexpressing cells showed an accelerated mitotic progression, which suggests that the αβ-tubulins-mediated regulation of GTP-bound status of Rheb might be an important for the proper mitotic progression. Therefore, we propose that soluble deacetylaed αβ-tubulins as a ...
Our data suggest that the bimodal actions of S1P on cell migration is due to receptor subtype-specific positive and negative regulation of Rho family GTPase, Rac; S1P 1 and S1P 3 mediate Rac stimulation and chemotaxis whereas S1P 2 mediates Rac inhibition and chemorepulsion. The stimulatory ...