IFA on HEp-2 cells was performed to screen the patients' sera for ANA and to determine the AC staining pattern. Immunoblot method was used to evaluate the specific ANA in patients' sera. We performed flow cytometric analysis of Th17 and Tregs' percentage in the peripheral blood of the ...
(IBST). The secondary antibody incubation was 1h at room temperature with 100µL of Cappel goat anti-cat IgGperoxidaseconjugate (MP Biomedicals, Santa Ana, CA) diluted 1:5000 in ELISA diluent with 5% mouse serum (Sigma, St. Louis, MO). Wells were again washed in a plate washer 5 ...
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