Moreover, IL-17A levels were elevated in the sera with AC-4 IFA pattern when compared to AC-29 (15.6 [4.4-28.9] vs 2.5[0.35-8.45], p=0.033). When opposing patients 'sera, positive for only one ANA, with "simple" AC-IFA pattern, to sera with mixed AC pattern (positive for more ...
Performances of anti-nuclear antibody testing by immunofluorescence assay (ANA-IFA) and enzyme immunoassay (ANA-EIA) were compared in relation to patient diagnosis. A total of 467 patient serum samples were tested by ANA-IFA (Kallestad; ... RA Gniewek,DP Stites,TM Mchugh,... - 《Clin Diag...
(IBST). The secondary antibody incubation was 1h at room temperature with 100µL of Cappel goat anti-cat IgGperoxidaseconjugate (MP Biomedicals, Santa Ana, CA) diluted 1:5000 in ELISA diluent with 5% mouse serum (Sigma, St. Louis, MO). Wells were again washed in a plate washer 5 ...
The occupational risk of forestry workers was the highest for Borrelia infection in comparison with other zoonoses (Hanta, lymphocytic choriomeningitis and tick-borne encephalitis viruses, Q-fever, leptospirosis, ehrlichiosis, anaplasmosis). The odds ratio was 17.0 for Borrelia seropositivity in a high...
ANA positivity was defined as a titer of ≥1:40, measured by an enzyme-linked immunosorbent assay (ELISA) on at least two occasions. In our practice, ELISA is used for initial screening, with positive results confirmed by HEp-2 cell indirect immunofluorescence (IFA) upon request to determine...
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