Real- time PCR was performed using FastStart Universal SYBR Green master mix (Roche Diagnostics GmbH, Mannheim, Germany) based on the manufacturer's protocol. Primers used for qRT-PCR were: CD44 5′-CATCTACCCCAGCAACCCTA-3′ (sense) 5′-ATCCCAGGTTTCTTGCCTCT-3′ (antisense) BMI1 5′-CAG...
so old-timey, so "Cheers-like" about an Irish pub. Back in the day the denizens of these friendly places were usually blue collar working men and women who stopped at their neighborhood Irish bar to chat and visit after getting out of the local factory...
so old-timey, so "Cheers-like" about an Irish pub. Back in the day the denizens of these friendly places were usually blue collar working men and women who stopped at their neighborhood Irish bar to chat and visit after getting out of the local factory...