H. (2009). Identification of polyubiquitin binding proteins involved in NF-kB signaling using protein arrays. Biochim. Biophys. Acta 1794, 1010-1016.Fenner BJ, Scannell M, et al. 2009. Identification of polyubiquitin binding proteins involved in NF-kB signaling using protein arrays. Biochimica et...
binding proteins (OTU), Machado–Joseph diseaseprotein domainproteases (MJD), MIU-containing novel DUB family (MINDY),monocytechemotactic protein-induced proteins (MCPIP) families, permutedpapainfoldpeptidaseofdsDNA virusesand eukaryotes (PPPDE), andzinc fingerwith UFM1-specific peptidasedomain protein(...
The ubiquitin polypeptide is conjugated via its C-terminus to amine groups of lysine residues on target protein. Additionally, ubiquitins moieties can be conjugated in tandem to the initial ubiquitin via any of its internal lysine residues or N terminal methionine residue, resulting in the formation...
Reveals, together with references 56 and 59, two binding sites for ubiquitin on NEDD4 family HECT domains. Article CAS PubMed PubMed Central Google Scholar Eisenhaber, B., Chumak, N., Eisenhaber, F. & Hauser, M.-T. The ring between ring fingers (RBR) protein family. Genome Biol. 8...
Protein | TwistDx | sigma aldrich | sigma | Life Technologies | Molecular Probes | gibco | invitrogen | thermo | Tonbo Biosciences | targetingsystems | sunrise sciene products | PEVIVA | MicroGEM | ModiQuest | LGC link | HILICON AB | Cellutron Life Technologies | Biomedical Research Service ...
& Dunn, R. Regulation of membrane protein transport by ubiquitin and ubiquitin-binding proteins. Annu Rev Cell Dev Biol 19, 141–72 (2003). 13. Thrower, J. S., Hoffman, L., Rechsteiner, M. & Pickart, C. M. Recognition of the polyubiquitin proteolytic signal. EMBO J 19, 94–102 ...
1. The same subset of animals was used for liquid chromatography mass spectrometry (LC/MS) to identify K48-polyubiquitin protein targets in whole cell protein lysates purified with a K48-specific tandem ubiquitin binding entity (K48-TUBE) (n = 6 animals per group per sex per age). Th...
Protein | TwistDx | sigma aldrich | sigma | Life Technologies | Molecular Probes | gibco | invitrogen | thermo | Tonbo Biosciences | targetingsystems | sunrise sciene products | PEVIVA | MicroGEM | ModiQuest | LGC link | HILICON AB | Cellutron Life Technologies | Biomedical Research Service ...
polyubiquitinated protein, comprising exposing the sample to at least one of the foregoing antibodies or antigen-binding fragments and determining the binding of the at least one antibody or antigen-binding fragment to a K63-linked polyubiquitin or K63-linked polyubiquitinated protein in the sample....
28 We supported this conclusion with preliminary SPR experiments that monitored the binding of one concentration of different Ub4 chains to the dual-UBA domain protein R23D (Figure 1a) immobilized by amine coupling at a high density.28 With such a high-density surface, the analyte may re-bind...