Human-human hybridomas have been produced by the method of the invention which sensitizes human tumor cells in the human spleen cells prior to fusion with human fetal marrow, human lymphoblastoid or human plasma cell lines. The monoclonal and polyclonal antibodies produced by the hybridomas ...
Two hybridomas were developed by fusing heteromyelomas to Epstein-Barr virus immortalized B cells obtained from beekeepers. The cloned hybridomas were stable and secreted up to 40 mg/L of antibody into the culture supernatant. Phospholipase A2 specificity of the human monoclonal antibodies was ...
Immunostimulating proteins, corresponding polyclonal and monoclonal antibodies, corresponding hybridomas for use in therapy and diagnosis. OF THE TECHNICAL CONTENT OF THE INVENTION The object of the invention is an immunostimulating glycoprotein having the following properties: a. it has a molecular mass...
Immunoblots were performed using hybridoma culture media of Kon 6 (1 A, B), Kon 7 (2A, B), and Tü 300 (3A, B) and by polyclonal antibody (4A, B). Staining was achieved as described in Materials and Methods. The IgG fraction of the rabbit serum was concentrated by ammonium ...
(spleen cell populations that include B cells) are fused with immortal myeloma tumor cells, resulting in the formation of immortalized hybridoma cells. Each hybridoma cell expresses the same antibody as its B-cell ancestor. A selection step using specialized culture media, in which only fused ...
These hybridomas have the ability to produce the antibody of interest in a similar manner to the antibody-secreting cells used in the fusion process. Also, the hybridomas are immortal cells as the myeloma cells used in the fusion are also immortal cells. Therefore the hybridomas are both ...
PEPTIDES AND POLYPEPTIDES FROM THE SUB-MAXILARY GLAND OF THE RAT, CORRESPONDING MONOCLONAL AND POLYCLONAL ANTIBODIES, CORRESPONDING HYBRIDOMAS AND APPLICATIONS OF SAID PRODUCTS TO THE DIAGNOSIS, DETECTION OR FOR THERAPEUTICAL PURPOSES 来自 掌桥科研 喜欢 0 阅读量: 8 申请(专利)号: WO1989FR00523 ...
TRF is shown to be distinct from IL 2 by the following observations: 1) culture supernatants from the cloned T cell line L2, collected over an 8-day period after allogeneic stimulation, transiently contain IL 2 activity but maintain high levels of TRF activity throughout 192 hr; 2) L2V, ...
b) fusing the spleen cell with a suitable fusion partner to form a hybridoma, and c) obtaining the monoclonal antibody from the hybridoma. Description: BACKGROUND OF THE INVENTION Invasive amebiasis, a disease caused by the enteric protozoan Entamoeba histolytica is a major public health problem ...
AllMouseRabbitRatGoatchickenSheepPurified From HEK 293 Cell culture Supernatant.Purified From Concentrated Hybridoma Tissue Culture Supernatant.CHO CellsDonkeyGuinea PigHamsterMouse-Balb/cArmenian HamsterMouOPN Knockout MousePigGolden Syrian HamsterCD8 Alpha, Leu-2aGaHumanHuman/Mouse ChimericRecombinantLlamaRabbi...