Functioning as a post-transcriptional regulation mechanism, APA modulates gene expression in eukaryotes [87]. APA can affect the stability and translation efficiency of the target mRNA. APA sites, which close to the stop codon, reduce the binding of potential regulatory elements, such as miRNAs and...
(A)-trimmed reads); ii) identification of candidate CPAS through peak calling and/or pileups at aligned read ends; iii) filtering—e.g., removal of CPAS candidates that are likely artifacts of internal Poly(A) priming, and/or of candidates lacking a nearby polyadenylation signal sequence (...
DNA sequences that read the same from 5 to 3 in either strand are called inverted repeat sequences or simply IRs. They are found throughout a wide variety of genomes, from prokaryotes to eukaryotes. Despite extensive research, their in vivo functions, if any, remain unclear. Using ...
Messenger RNA polyadenylation in eukaryotes marks the end of a transcript, and the process is associated with transcription termination. Increasing evidence reveals the potential of gene expression regulation through alternative polyadenylation. The site of poly(A) addition is defined by poly(A) signals...
Mandel, C. R., Bai, Y. & Tong, L. Protein factors in pre-mRNA 3′-end processing.Cell. Mol. Life Sci.65, 1099–1122 (2008). ArticleCASPubMedPubMed CentralGoogle Scholar Zhao, J., Hyman, L. & Moore, C. Formation of mRNA 3′ ends in eukaryotes: mechanism, regulation, and inter...
Novel methods to sequence RNA 3′ ends have generated comprehensive catalogues of polyadenylation (poly(A)) sites; their analysis using innovative computational methods has revealed how poly(A) site choice is regulated by core RNA 3ʹ end processing factors, such as cleavage factor I and ...
over half also possessed AATAAA. In contrast, only ~10% of bins that did not contain a polyA site had the AATAAA signal. This enrichment was observed for all binary features, i.e., all the DNA sequence features and the original transcriptome end location feature (Supplementary Fig.1a). The...
At high dilutions and in the absence of the specificity factor, the enzyme is almost completely inactive and indifferent to the presence or absence of the AAUAAA sequence in the substrate RNA. When complemented with CPSF, the enzyme acts only on RNAs carrying the AAUAAA signal. A CPSF may ...
Plant polyadenylation signals in general are more similar to yeast, in which no highly conserved signal sequences have been identified. For example, a recent work revealed that the NUE signal AAUAAA, albeit proven the best signal in plants [7], can only be found at the right position in abo...
The cis-acting signal sequences required for mRNA 3 end formation are highly conserved and well characterized in higher eukaryotes. However, the situation ... S Irniger,GH Braus - 《Proceedings of the National Academy of Sciences of the United States of America》 被引量: 119发表: 1994年 ...