(E) scPolyA-seq 的reads更多地集中在mRNA的3′端。 通过分析多个细胞系的 scPolyA-seq,研究团队发现大量细胞数据(bulk data)中使用了多个polyA位点的基因倾向于在单细胞层面仅使用一个polyA位置。有趣的是,对 scPolyA-seq数据分析发现polyA位点使用高度变异的基因列表中富集了细胞周期相关基因。研究团队进一步发现细...
The template preparation protocol determines the comparability of ribo-depleted RNA-Seq to polyA RNA-Seq.Ru, HuangMarkus, JaritzPhilipp, GuenzlIrena, VlatkovicAndreas, SommerIdo, M. TamirHendrik, MarksThorsten, KlampflRobert, KralovicsHendrik G., Stunnenberg...
在本次网络研讨会上,BGI中国研发总监唐冲博士将描述他如何使用PacBio Iso-Seq方法评估单个mRNA分子的poly(A)尾部长度。该方法能支持研究人员评估多聚腺苷酸化长度对单分子水平转录物的功能影响。 唐冲博士还将分享一种新的基于PacBio SMRT测序的建库流程,可将Iso-Seq测序通量提高3-4倍。 Assessing Polyadenylation L...
To allow efficient transcript/gene detection, highly abundant ribosomal RNAs (rRNA) are generally removed from total RNA either by positive polyA+ selection or by rRNA depletion (negative selection) before sequencing. Comparisons between the two methods have been carried out by various groups, but th...
Ribo-depleted RNA-Seq detects macro ncRNAs more efficiently than polyA RNA-Seq.Ru, HuangMarkus, JaritzPhilipp, GuenzlIrena, VlatkovicAndreas, SommerIdo, M. TamirHendrik, MarksThorsten, KlampflRobert, KralovicsHendrik G., Stunnenberg
PT_identify_polyA_sites is a Python-based tool designed to identify and analyze poly(A) sites in RNA-seq data. This tool processes BAM files to extract poly(A) sites and performs statistical analysis to compare the poly(A) site distributions between wild-type (WT) and mutant (MUT) samples...
紧急救助大虾！！能不能通过cufflinks软件处理RNAseq数据获得的转录本信息得到此转录本polyA位点么？如果...
Quartz-Seq RamDA: Extremely Reproducible Single-Cell Non-polyA mRNA Sequencing 来自 confit.atlas.jp 喜欢 0 阅读量: 68 作者: I Nikaido 年份: 2015 收藏 引用 批量引用 报错 分享 全部来源 求助全文 confit.atlas.jp 研究点推荐 Quartz-Seq RamDA ...
R package for visualization and exploratory analysis of Oxford Nanopore direct RNA seq based polyA predictions - smaegol/nanotail
Seq-Star™ poly(A) mRNA Isolation Kit AS-MB-006-01 24 reactions Seq-Star™ poly(A) mRNA Isolation Kit AS-MB-006-02 96 reactions 产品特点 1. 双次mRNA结合方案有效满足高通量RNA测序文库构建对mRNA的高纯度要求 2. 优化的试剂盒体系及高质量磁珠保证了高mRNA回收效率 3. 操作简单,无需...