RNA polymerase IIPoly(A) RNAPollen grainHyacinthus orientalisSpatial distribution of poly(A) RNA, hypophosphorylated Pol IIA, and hyperphosphorylated Pol IIO form of polymerase RNA II was characterized using immunofluorescence, immunogold and fluorescence in situ hybridization (FISH) techniques in ...
产品类型:RNA-Seq建库 品牌:Lucigen 货号:PAP5104H 规格:50 Rxns 价格:3271 产品介绍 The Poly(A) Polymerase Tailing Kit was developed for the rapid and efficient addition of poly(A)-tails to the 3′-hydroxyl end of any RNA. RNA with 3′ phosphates may be endrepaired using T4 Polynucleotide ...
图1. 鉴定了一个OIS相关的非编码RNA—EPB41L4A-AS1,其编码SNORA13 接下来,深入研究了SNORA13参与衰老的分子机制。细胞分离和FISH实验证明,SNORA13定位于核仁,且致癌基因诱导后不影响该snoRNA的定位及整体表达。通过多种分析证实了SNORA13是18S.1248U假尿苷化所必需的。由于18S:1248U位点上m1acp3Ѱ修饰在真核...
因此,SNORA13通过非典型的分子机制调节核糖体生成和p53通路,这种机制与其指导RNA修饰的作用不同。这些发现扩展了我们对snoRNA功能及其在细胞信号传导中作用的理解。 【主要实验】 CRISPRi筛选、RNA荧光原位杂交(FISH)、嘌呤霉素掺入实验、核糖体分析(Ribo-seq、Polysome profiling)、RNA-seq、ChIP实验、质谱分析、UV-RI...
图1.鉴定了一个OIS相关的非编码RNA—EPB41L4A-AS1,其编码SNORA13 接下来,深入研究了SNORA13参与衰老的分子机制。细胞分离和FISH实验证明,SNORA13定位于核仁,且致癌基因诱导后不影响该snoRNA的定位及整体表达。通过多种分析证实了SNORA13是18S.1248U假尿苷化所必需的。由于18S:1248U位点上m1acp3Ѱ修饰在真核...
Ribo-seq数据显示前2个外显子与核糖体相关,但PhyloCSF评分未显示这些区域有任何编码潜力(图1I)。这些结果表明,Cardinal是一种非编码RNA。 FISH实验中显示:在HL-1细胞和分离的成年小鼠心肌细胞的核室和细胞质室中均检测Cardinal(图1J和1K)。定量显示,在HL-1细胞和成人心肌细胞中,超过60%的Cardinal信号位于细胞质中...
C Analysis of the expression of CXCL2 in PARP inhibitor Ola or p38 inhibitor SB203580 treated cells using a RNA FISH assay. Cells were treated with TNFα for 1 h in the absence or presence of Ola or SB.The levels of CXCL2 mRNA were determined by fluorescence in situ hybridization (red...
RNA modifications are important regulators of gene expression1. In Trypanosoma brucei, transcription is polycistronic and thus most regulation happens post-transcriptionally2. N6-methyladenosine (m6A) has been detected in this parasite, but its function
（1）在荧光原位杂交（FISH）过程中，将PSP与目标RNA结合，通过荧光显微镜观察。（2）使用滚环扩增（RCA）技术制备PSP，使其与目标核酸形成较高的亲和力结合，从而实现单分子灵敏度检测。多硫化氢（Hydrogen Polysulfide, H2Sn）检测：PSP探针也可用于检测细胞中的多硫化氢水平，其检测方法包括：（1）使用双光子荧光探针...
Bleckmann, A. & Dresselhaus, T. Whole mount RNA-FISH on ovules and developing seeds. Methods Mol. Biol. 1669, 159–171 (2017). Article CAS Google Scholar Zimmerberg, J. & Whitaker, M. Irreversible swelling of secretory granules during exocytosis caused by calcium. Nature 315, 581–584 ...