*1 pMD®18-T Vector的使用量 取 0.5 μl实验也可得到满意的结果。实际操作时,可按实验需要确定T载体的使用量。pMD®18-T Vector 1 μl(50 ng)的摩尔数约为 0.03 pmol。 *2 Control Insert Control Insert 为 500 bp 的 3′末端带有 A 碱基的 PCR 产物,Control Insert 1 μl(50 ng)的摩 尔...
pMDr18-T Vector* 11plControl Insert*21pldH2032)加入 5 pl (等童)的 Solution I.3)16P反应30分钟.注)室温(259也能正常进行连接反应,但反应效率稍微降低。5分钟也能正常进行连接反应,但反应效率稍微降低。4)全H (10 pl)加入至100MJM109燼受态细胞中,冰中SJt 30分伊5)42P加热45秒钟后,再在冰中放...
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RestrictionanalysisonDNAMAN1Methylation:dam-Nodcm-NoScreenedwith1364enzymes809sitesfoundAacI1G/GATCC436AaeI1G/GATCC436AaqI3G/TGCAC177117373AatII1GACGT/C68AcaII1G/GATCC436AcaIIITGC/GCA58198Acc113I1AGT/ACT186Acc16ITGC/GCA58198Acc65I1G/GTACC445AccBSI3GAG/CG
pMD18-T simple vector图谱序列_生物学_自然科学_专业资料。pMD18-T simple vector 图谱序列 pMD18-T simple Vector 1 TCGCGCGTTT CGGTGATGAC GGTGAAAACC TCTGACACAT GCAGCTCCCG 51 GAGACGGTCA CAGCTTGTCT GTAAGCGGAT GCCGGGAGCA GACAAGCCCG 101 TCAGGGCGCG TCAGCGGGTG TTGGCGGGTG TCGGGGCTGG CTTAACTATG ...
pMD18-T酶切位点及序列.doc,Restriction analysis on DNAMAN1 Methylation: dam-No dcm-No Screened with 1364 enzymes, 809 sites found AacI 1 G/GATCC 436 AaeI 1 G/GATCC 436 AaqI 3 G/TGCAC 177 1127 2373 AatII 1 GACGT/C 2628 AcaII 1 G/GATCC 436 AcaIII 2 TGC/GCA
pMD18-T酶切位点及序列 Restriction analysis on DNAMAN1 Methylation: dam-No dcm-No Screened with 1364 enzymes, 809 sites found AacI 1 G/GATCC 436 AaeI 1 G/GATCC 436 AaqI 3 G/TGCAC 177 *** *** AatII 1 GACGT/C 2628 AcaII 1 G/GATCC 436 AcaIII 2 TGC/GCA 258 1928 Acc113I 1...
pMD18-T酶切位点及序列..doc,Restriction analysis on DNAMAN1 Methylation: dam-No dcm-No Screened with 1364 enzymes, 809 sites found AacI 1 G/GATCC 436 AaeI 1 G/GATCC 436 AaqI 3 G/TGCAC 177 1127 2373 AatII 1 GACGT/C 2628 AcaII 1 G/GATCC 436 AcaIII 2 TGC/GCA
pMD18-TVectorPositionsofvariouselementsVectorsize(bp)2692Multiplecloningsite399-461CloningSite425LacZα-peptide146-475Ampicillinresistance..