pMD18-TsimpleVector1TCGCGCGTTTCGGTGATGACGGTGAAAACCTCTGACACATGCAGCTCCCG51GAGACGGTCACAGCTTGTCTGTAAGCGGATGCCGGGAGCAGACAAGCCCG101TCAGGGCGCGTCAGCGGGTGTTGGCGGGTGTCGGGGCTGGCTTAACTATG151CGGCATCAGAGCAGATTGTACTGAGAGTGCACCATATGCGGTGTGAAATA01CCGCACAGATGCGTAAGGAGAAAA
pMD18-TVectorPositionsofvariouselementsVectorsizebp69Multiplecloningsite399-461CloningSite45LacZα-peptide146-475Ampicillinresistancegene163~49pUCorigin873-1461primerbindingsites:BcaBESTSequencingPrimerM13-47bindingsite35-375BcaBESTSequ...
*1 pMD®18-T Vector的使用量 取 0.5 μl实验也可得到满意的结果。实际操作时,可按实验需要确定T载体的使用量。pMD®18-T Vector 1 μl(50 ng)的摩尔数约为 0.03 pmol。 *2 Control Insert Control Insert 为 500 bp 的 3′末端带有 A 碱基的 PCR 产物,Control Insert 1 μl(50 ng)的摩 尔...
pMD18-T simple vector图谱序列_生物学_自然科学_专业资料。pMD18-T simple vector 图谱序列 pMD18-T simple Vector 1 TCGCGCGTTT CGGTGATGAC GGTGAAAACC TCTGACACAT GCAGCTCCCG 51 GAGACGGTCA CAGCTTGTCT GTAAGCGGAT GCCGGGAGCA GACAAGCCCG 101 TCAGGGCGCG TCAGCGGGTG TTGGCGGGTG TCGGGGCTGG CTTAACTATG ...
pMD18-TsimpleVectorpMD18-Tsimplevector图谱序列pMD18-TsimpleVector1TCGCGCGTTTCGGTGATGACGGTGAAAACCTCTGACACATGCAGCTCCCG51GAGACGGTCACAGCTTGTCTGTAAGCGGATGCCGGGAGCAGACAAGCCCG101TCAGGGCGCGTCAGCGGGTGTTGGCGGGTGTCGGGGCTGGCTTAACTATG151CGGCATCAGAGCAGATTGTACTGAGAGTGC雪酗瘟溅又瑞乙鬼毯勤袜剥闷秽依谰呆榆荫...
TaKaRa Code: D101ApMD174;T VectorTaKaRa宝生物工程大连有限公司内容Page制品说明1制品内容1保存1纯度1用途1 pMD174;18T Vector的结构1实验操作2Control DNA片段的克隆实验
pMD18-TsimpleVectorpMD18-Tsimplevector图谱序列pMD18-TsimpleVector1TCGCGCGTTTCGGTGATGACGGTGAAAACCTCTGACACATGCAGCTCCCG51GAGACGGTCACAGCTTGTCTGTAAGCGGATGCCGGGAGCAGACAAGCCCG101TCAGGGCGCGTCAGCGGGTGTTGGCGGGTGTCGGGGCTGGCTTAACTATG151CGGCATCAGAGCAGATTGTACTGAGAGTGC锹娶酷清阀酝稀翟蔽宣懈孰堕鼓洪离钻擦户...
pMD18-T酶切位点及序列 Restriction analysis on DNAMAN1 Methylation: dam-No dcm-No Screened with 1364 enzymes, 809 sites found AacI 1 G/GATCC 436 AaeI 1 G/GATCC 436 AaqI 3 G/TGCAC 177 *** *** AatII 1 GACGT/C 2628 AcaII 1 G/GATCC 436 AcaIII 2 TGC/GCA 258 1928 Acc113I 1...
pMD18-T Vector Positions of various elements Vector size (bp) 2692 Multiple cloning site 399-461 Cloning Site 425 LacZ α-peptide 146-475 Ampicillin resistance gene 1632~2492 pUC origin 873-1461 primer binding sites: BcaBEST Sequencing Primer M13-47 binding site 352-375 BcaBEST Sequencing Prime...
pMD18-T酶切位点及序列..doc,Restriction analysis on DNAMAN1 Methylation: dam-No dcm-No Screened with 1364 enzymes, 809 sites found AacI 1 G/GATCC 436 AaeI 1 G/GATCC 436 AaqI 3 G/TGCAC 177 1127 2373 AatII 1 GACGT/C 2628 AcaII 1 G/GATCC 436 AcaIII 2 TGC/GCA