PMA-induced biosynthetic changes in differentiating muscle cells in culturedoi:10.1016/0309-1651(82)90034-0B.M. Zani and M. MolinaroCell Biology International Reports
160ml cell culture flasks (Nunc, Wiesbaden, Germany). For differentiation, the cells were cultivated in 24-well plates (Nunc) at a density of 5×104 cells/ml/well and stimulated with PMA (Sigma–Aldrich, Seelze, Germany) at a concentration of 0.1ug/ml for 2 days (days −2 to day ...
In v-PCR, pretreatment of a cell culture with a viability dye such as PMA allows differentiation of live and dead cells using qPCR or other DNA amplification methods (Figure 4). v-PCR has been validated in a wide variety of bacterial strains, as well as yeasts, fungi, viruses, and ...
et al. Influence of tumor cell culture supernatants on macrophage functional polarization: in vitro ...
图2 细胞表面标志CD11b、CD14表达Figure 2 Expression of cell surface markers CD11b and CD14 其次分别用10 ng/ml和50 ng/ml PMA处理THP-1细胞,发现正常培养条件下即对照组THP-1细胞CD14、CD11b表达量不高,而有报道称,PMA、TPA等可诱导未成熟的髓系细胞,向巨噬细胞方向分化,并使单核分化标志物CD11b和CD14...
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This modification process will make DNA insoluble and make it lost together with cell debris in the subsequent genomic DNA extraction process, thus hindering the PCR amplification of target DNA in dead cells. The unbound PMA remaining in the solution reacts with water molecules under strong light ...
We now offer a broad range of Cell Culture Reagents. Cell Culture Reagents include a multitude of compounds, from solvents and nutrients to growth promoters and apoptosis inducers. Our popular products such asDimethyl Sulfoxide (DMSO), Cell Culture reagent,PMA, andSodium Pyruvate Solution 100 mM ...
Cell culture BEAS-2B human bronchial epithelial cells (American Type Culture Collection, ATCC CRL 9609), were grown in RPMI1640 supplemented with 10% FBS, 0.1 mM non-essential amino acids, penicillin (50 U/ml) and streptomycin (50 g/ml) at 37oC in a humidified 95%/5% (v/v) mixture ...
Food Ingredients from Plant Cell and Tissue Culture Those that are not GRAS are defined as "food additives" under the FFDCA and are subject to premarket review and approval by the Food and Drug Administration (FDA) before they can be used in food. Before any new color additive ... N Beru...