Neural cells differentiated from pluripotent stem cells(PSCs), including both embryonic stem cells and induced pluripotent stem cells, provide a powerful tool for drug screening, disease modeling and regenerative medicine. High-purity oligodendrocyte progenitor cells(OPCs) and neural progenitor cells(NPCs...
多能干细胞(Pluripotent Stem Cells) 简介 如前所述,人多能干细胞 (PSC) 的特点是能够无限制自我更新,当遇到合适的环境时,可以分化成人体的几乎任意细胞类型。常用于研究的两种 PSC 是胚胎干细胞 (ESC) 和诱导 PSC(iPSC) ( 图2)。人 ESC 分离自发育胚胎的胚泡期内细胞群1。 iPSC 与 ESC 类似或相当,但通...
事实上,在体外没有转基因诱导的情况下,通过定向分化从PSCs获得可植入的HSCs仍然是一个难题103。 我们研究团队的目标是解锁通过PSCs定向分化衍生的HSPC的体内移植能力,以改进疾病建模和推进基于iPSC的造血治疗。基于小鼠胚胎干细胞来源的原始HSPC可以通过强制表达同源异型转录因子HOXB4(reFS104,105)而被重新鉴定为具有多...
Xu等人(375)首先展示了一种用于人类PSCs培养的Ff系统,其方法是用基质胶(Matrigel)取代MEF饲养层,基质胶本身是一种异种基质,并使用层粘连蛋白作为粘合剂。其他材料,如重组蛋白和合成聚合物也可以取代饲养层细胞(40,190,209,219,281)。使用人饲养层细胞和层粘连蛋白基质的Xf条件也被描述过(87,177)。Thomson团队报告...
IntroductionPluripotent stem cells (PSCs) are promising tools for modern regenerative medicine applications because of their stemness properties, which include unlimited self-renewal and the ability to differentiate into all cell types in the body. Evidence suggests that a rare population of cells withi...
Essential 8™ Medium is a fully defined, feeder-free medium formulated for the growth and expansion of human pluripotent stem cells (PSCs). Originally developed by Chen et al.1 in the laboratory of James Thomson, and validated by Cellular Dynami...
Human pluripotent stem cells (PSCs) can give rise to any cell type in the body. PSC derivatives are being developed for therapeutic use in diseases affecting organs and organ systems such as the eye, nervous system, heart, pancreas, liver, intestines, lung, circulatory system and skin, as ...
本发明涉及包含作为从鸟类的蛋(egg)分离的细胞的胚盘多能干细胞(pluripotent stem cells,PSCs)或神经干细胞(neural stem cell s,NSCs),胚胎成纤维细胞(embryonic fibroblasts,FBs)条件培养基(conditioned medium)作为有效成分的用于细胞增殖,皮肤再生及皱... 李廷福 被引量: 0发表: 2020年 人胚胎干细胞分化为可自...
Pluripotent stem cells (PSCs) are a valuable tool for interrogating development, disease modelling, drug discovery and transplantation. Despite the burgeoned capability to fate restrict human PSCs to specific neural lineages, comparative protocols for mouse PSCs have not similarly advanced. Mouse protoc...
The maintenance and expansion of undifferentiated pluripotent stem cells (PSCs) in cell culture systems requires the dissociation of cells and their transfer from culture vessel to culture vessel, in a process known as passaging. This chapter includes images acquired from different techniques used to...