pLKO.1 Stuffer-containing lentiviral vector for expression of shRNA. This plasmid is the official TRC cloning vector. Sequence Author: The RNAi Consortium Open in SnapGene Try SnapGene for Free Download Plasmid|Download SnapGene Viewer Explore Over 2.7k Plasmids: Viral Expression & Packaging Vectors ...
CONCLUSION:The lentiviral knockdown plasmid pLKO.1-hSRF was successfully constructed and the SAS cell line of low expression of SRF was obtained.【KEY WORDS】lentiviral vector;plasmid construction;serum response factor;oral squamous cell carcinoma 构建敲减人血清反应因子 基因的慢病毒质粒,为进一步研究SRF...
产品介绍 质粒名称:TET-pLKO.1 PURO shLuc 质粒编号:83092 质粒备注: Lentiviral shRNA vector for inducible knockdown of luciferase (control hairpin) 载体名称:Tet-pLKO-puro 载体大小:10633 完整质粒大小:8816 载体类型:哺乳动物表达,慢病毒 筛选标记:嘌呤霉素 ...
mission lentiviral plasmids shctrl plko 1 puro(Millipore) 86 Milliporemission lentiviral plasmids shctrl plko 1 puro Mission Lentiviral Plasmids Shctrl Plko 1 Puro, supplied by Millipore, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, ar...
A.2 Map of pLKO.1 pLKO.1 is a replication-incompetent lentiviral vector chosen by the TRC for expression of shRNAs. pLKO.1 can be introduced into cells via direct transfection, or can be converted into lentiviral particles for subsequent infection of a target cell line. Once introduced, the...
Our results showed that the newly constructed pLKOG plasmid, as a lentiviral vector carrying shRNA, could knock down the target gene expression efficiently and express TurboGFP protein efficiently in the host cells. We conclude that the new plasmid is a convenient vector for selecting positive ...
Please cite Moffat et al., Cell 2006 Mar; 124(6):1283-98 (PubMed) in all publications arising from the use of this vector. A.2 Map of pLKO.1 pLKO.1 is a replication-incompetent lentiviral vector chosen by the TRC for expression of shRNAs. pLKO.1 can be introduced into cells via ...
r C.3 Digesting pLKO.1 TRC-Cloning Vector r C.4 Ligating and transforming into bacteria r D.1 Recommended materials r D.2 Screening for inserts r E.1 Recommended materials r E.2 Protocol for producing lentiviral particles r F.1 Recommended materials r F.2 Determining the optimal puromycin...
价格:询价 载体描述: pLKO.1-puro is a transfer and cloning vector of shRNA, originally derived from HIV-1. It contains all the necessary cis-elements for packaging, reverse transcription, and integration, which are required for subsequent production of the lentiviral particles. ...
A.2 Map of pLKO.1 pLKO.1 is a replication-incompetent lentiviral vector chosen by the TRC for expression of shRNAs. pLKO.1 can be introduced into cells via direct transfection, or can be converted into lentiviral particles for subsequent infection of a target cell line. Once introduced, the...