Puro 荧光标记: ■质粒简介 pLKO.1-Puro is a cloning vector of shRNA, originally derived from HIV-1. It contains all the necessary cis-elements for packaging, reverse transcription, and integration for subsequent production of the lentiviral particles. ...
pLKO.1 puro 2ug pLKO.1-puro is a transfer and cloning vector of shRNA, originally derived from HIV-1. It contains all the necessary cis-elements for packaging, reverse transcription, and integration, which are required for subsequent production of the lentiviral particles. ...
产品介绍 质粒名称:TET-pLKO.1 PURO shGFP 质粒编号:83085 质粒备注: Lentiviral shRNA vector for inducible knockdown of GFP (control hairpin) 载体名称:Tet-pLKO-puro 载体大小:10633 完整质粒大小:8818 载体类型:哺乳动物表达,慢病毒 筛选标记:嘌呤霉素 ...
Lentiviral shRNA vector for inducible knockdown of luciferase (control hairpin) Tet-pLKO-puro(pLKO-Tet-ON) Backbone size w/o insert (bp)10633 Total vector size (bp)8775 Vector type Mammalian Expression, Lentiviral, RNAi Selectable markers Puromycin Comprehensive genomic analysis identifies SO...
vector_types Mammalian Expression Lentiviral RNAi growth strain : 3rd gen lentiviral backbone for cloning and expression of new shRNA sequences. Uses puromycin for selection. origin : 37 pi : alt_names LKO.1 cloning clone_methodRestriction Enzyme ...
真核抗性:Puro+ 培养条件:37℃ 表达宿主:哺乳细胞 诱导方式:无须诱导,瞬时表达 5'测序引物:U6-5(5'-ATGGACTATCATATGCTTACCGTA-3') 3'测序引物:Sv40-polyA-R(5'-GAAATTTGTGATGCTATTGC-3') 载体介绍 Lentiviral expression vector for shRNA expression (U6 promoter); amp resistance (puromycin for mammal...
价格:询价 载体描述: pLKO.1-puro is a transfer and cloning vector of shRNA, originally derived from HIV-1. It contains all the necessary cis-elements for packaging, reverse transcription, and integration, which are required for subsequent production of the lentiviral particles. ...
3.Protocol:pLKO.1puro DavideGabellini 3.1MapofpLKO.1puro Fig2:MapofpLKO.1containinganshRNAinsert.TheoriginalpLKO.1-TRCcloning vectorhasa1.9kbstufferthatisreleasedbydigestionwithAgeIandEcoRI(seelater). Table1.DescriptionofpLKO.1-TRCcloningvectorelements. ...
A.2 Map of pLKO.1 pLKO.1 is a replication-incompetent lentiviral vector chosen by the TRC for expression of shRNAs. pLKO.1 can be introduced into cells via direct transfection, or can be converted into lentiviral particles for subsequent infection of a target cell line. Once introduced, the...
A.2 Map of pLKO.1 pLKO.1 is a replication-incompetent lentiviral vector chosen by the TRC for expression of shRNAs. pLKO.1 can be introduced into cells via direct transfection, or can be converted into lentiviral particles for subsequent infection of a target cell line. Once introduced, the...