IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight. Applications Key W: Western Blotting Cross-Reactivity Key H: Human M: Mouse Trademarks and Patents Cell Signaling Tech...
WB result of PLK4 antibody (12952-1-AP, 1:2000) with si-control and si-PLK4 transfected A549 cells.View All Images (10) WB analysis using 12952-1-AP mouse lung tissue were subjected to SDS PAGE followed by western blot with 12952-1-AP (PLK4 antibody) at dilution of 1:500 incubated...
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The secondary antibody incubations were carried out at room-temperature for 1 hour, and washes were performed with PBS. Then coverslips were dried and then mounted on glass slides using a mounting medium containing DAPI (Vector Laboratories; H-1000-10). The primary antibodies and their ...
For all immunoblotting analyses, the lysates were immunoblotted with anti-b-actin antibody as a loading control. Indirect immunofluorescence Cells were washed in PBS, and fixed with cold methanol for 20 min. The cells were incubated with 10% normal goat serum in PBS for 1 h at room ...
(Fig.2a). Moreover, the generated antibody against Plk4 (Fig.S2d) also closely co-localized with Cep131 at the centriole (Fig.2b). As previously reported12,33, most cells exhibited centriolar Plk4 localized in a dot-like pattern on the outer wall of the centriole, while the Cep131 ...
(R&D Systems) | seven hills bioreagents | zytovision | zyagen Laboratories | ZeptoMetrix | WSU Monoclonal Antibody Center | VMRD | VitaNavi | Virusys | Usbiological | UBPBIO | tulip biolabs | TriLink BioTechnologies | TRC | Ted Pella | swant | steraloids | ST john's laboratory | Sphero...
and cell lysates were mixed to a volume of 400 µl. The cell lysates obtained from the cells were precleared by incubation for 2 h at 4 °C on a shaker. The clear supernatant was incubated overnight with GFP, anti-GFP (ProteinTech Group, Wuhan) or anti-FLAG antibody (...
The rabbit PLK4ac antibody was used at a 1/500 or at 1/5,000 dilutions in the different applications (as indicated). Inducible HeLa shRNA cell lines. Generation of the inducible HeLa cells harbouring shRNA directed either against GCN5/KAT2A (target sequences: 50-CGTGCTGTCACCTCGAATGA-...
(R&D Systems) | seven hills bioreagents | zytovision | zyagen Laboratories | ZeptoMetrix | WSU Monoclonal Antibody Center | VMRD | VitaNavi | Virusys | Usbiological | UBPBIO | tulip biolabs | TriLink BioTechnologies | TRC | Ted Pella | swant | steraloids | ST john's laboratory | Sphero...