(PLD) lipid-signaling enzyme superfamily has long been studied for its roles in cell communication and a wide range of cell biological processes. Potent PLD2 inhibitors are available, like VU 0364739 hydrochloride (Tocris Cat. 4171) and ML 298 hydrochloride (Tocris Cat. 4895). Three different ...
This antibody detects only the cleaved matrix protein MAp17 and therefore labels mature virus particles. MAp17 signals were found at the plasma membrane and were barely detectable inside the cell, indicating that viral budding mainly occurs at the plasma membrane. The colocalization between Env and...
We describe here three methods for comprehensively assaying those phospholipases: 1) in vitro microassays, in which a radiolabeled substrate is exogenously added to cell lysates to measure the enzyme activity(ies); 2) immunocomplex assays, in which immunoprecipitation with a specific antibody is ...