PLGA-Resveratrol NPs optimal synthesis, using as input variables the amount of PLGA, Resveratrol, and PVA concentration, yield a hydrodynamic diameter of 137 nm, polydispersity index of 0.195, drug loading of 16 %, and z-potential of -24.71 mV. These results are in very close agreement with ...
The antibodies were diluted 1:1000 (according to the manufactures protocol) in 5% BSA in TBS-T. After washing with TBS-T, the membranes were incubated for 2 h at room temperature with goat anti-rabbit horseradish peroxidase (HRP) conjugated secondary antibody (Cell Signaling Technology, Inc.)...
Dynamic light scattering was used to confirm nanoparticle size after each ultracentrifugation step. Ultimately, this resulted in a protocol that allows for the isolation of nanoparticles of consistent size across synthetic batches without the presence of free EtNBS. Preparation of chemically-conjugated ...
Millipore, Burlington, MA, USA). The apoptotic detection kit was used according to the manufacturer’s protocol. In brief, the experiment was performed in a 6 well plate. The
The protocol for hEnSC extraction from the endometrium in this study has been previously reported [31, 32]. Flow cytometry analysis was performed to confirm the purity of the isolated stem cell cells. The list of antibodies that were used for flow cytometry in this study is as follows: CD...
cDNA synthesis 2μg of total RNA was performed with Revert Aid First strand cDNA synthesis Kit (Fermentas, USA) according to the manufacturer’s protocol. 20 μL of reaction volume was performed with iCycler thermal cycler (Bio-Rad; Hercules, CA) at 25 °C for 5 min, 42 °C for 60 ...
O. Ishola, S. O. Fakayode and I. M. Warner, “Experimental Design and Multivariate Analysis for Optimizing Poly (D,L-Lactide-co-Glycolide) (PLGA) Nanoparticle Synthesis Using Molecular Micelles,” Journal of Nanoscience and Nanotechnology, Vol. 8, No. 1, 2008, pp. 280-292....
cDNA synthesis 2μg of total RNA was performed with Revert Aid First strand cDNA synthesis Kit (Fermentas, USA) according to the manufacturer’s protocol. 20 μL of reaction volume was performed with iCycler thermal cycler (Bio-Rad; Hercules, CA) at 25 °C for 5 min, 42 °C for 60 ...
Given the importance of PLGA NPs and their widespread use, we consider these results of relevance for future work regarding PLGA nanoparticle use in metabolically-compromised clinical and experimental settings. Methods PLGA NP synthesis and delivery...
The cell-extracellular matrix (ECM) interactions are known to have a strong impact on cell behaviors in neural tissues. Due to complex physiology system and limited regenerative capacity of nervous system, neural tissue engineering has attracted attention as a promising strategy. In this study, we ...