For determination of the in vitro effects of the PLC inhibitor U73122 (Cat. No: U 6756, Sigma), HUVEC cells were treated with10 μ M for 24 h at 37 °C followed by 50 μ g/mL LPS for another 24 h., followed by qRT-PCR analysis. References 1. Burns, J. C. ...
Inhibitor concentrations and suppliers are as follows: d-erythro-sphingosine C-18 (2.5 μM; Caymanchem; in DMSO); Calphostin C (200 nM; Santa Cruz Biotechnology; in DMSO); U73122 (4.5–7.5 μM; Caymanchem; in DMSO); blebbistatin (25 μM; Sigma-Aldrich; in DMSO); ML-7 (...
MethodsPC12 cells were exposed to 50 μmol/L H 2 O 2 after pretreatment with 10 μmol/L U73122, a specific PLC-γ1 inhibitor. Hoechst/PI double staining was performed to observe the morphological changes of the cells under light microscope. MTT assay was used to evaluate the cell ...
However, this activation was significantly inhibited by pretreatment with PTX or U73122, a PLC inhibitor (Fig. 5B), indicating that SST-induced PLC activation is mediated by the Gαi/o pathway. Based on these results, we next assessed the possible roles of PDZK1 and PLC-β3 in SST-...
UTP经PLC-IP3信号通路调控猪冠状动脉平滑肌细胞自发性瞬时外向电流.pdf,维普资讯 生理学报ActaPhysiologicaSinica,February25,2008,60(1):65—73 http://www.actaps.corn.ca UTP经PLC.IP3信号通路调控猪冠状动脉平滑肌细胞 自发性 瞬时外 向电流 李鹏云,曾晓荣
RnasinRibontmleaseInhibitor GoldenEASYPCR system 1.4试剂配制 Oxoid公司 日本进口分装 进口分装 Bebco公司 Bebco公司 进口分装 进口分装 进口分装 英国Whatman公司 Sigma公司 美国Oenview公司 北京天为时代公司 大连宝生物公司 美国Invitrogen公司 TaKaRa公司 NEB公司 ...
3.1. Effect of PLCγ1 inhibitor U73122 on differentiation capacity of rat BMSCs After treated with PLCγ1 inhibitor U73122 for different days, the cell viabilities of BMSCs were measured using the MTT assay. Except 0.5 day, the cell viabilities in U73122-treated BMSCs declined significantly...
Incubation of HGC27 WISP-2 kd cells with both 0.75 μ M (U73122) and 1.125 μ M (STK870702) of PLC-γ small inhibitors and 1.5 μ M of JNK small inhibitor diminished such effect. (D) Incubation of HGC27 WISP-2 kd cells with FAK small inhibitor had no effect on cell ...
(N) TRPC1α/4β and (O) TRPC1α/5 currents increased continuously upon CCh perfusion and pretreatment with 20 μM U73122, which is a PLC inhibitor. (P) Summary of the reduction in the inactivation ratio for the TRPC1α/4β and TRPC1α/5 currents caused by U73122 and U73433 ...
(B,C) BJ fibroblast cells were treated with the PI3K inhibitor LY294002 (B) or the PLCγ inhibitor U73122 (C) and contraction proceeded in presence or absence of 20 ng/mL PDGF-BB. In all panels, values represent average of at least four independent experiments. (*) indicates p <...