HomogeneousformatAgarose gel electrophoresis with subsequent DNA extraction from gel is routinely used for DNA fragment isolation after plasmid DNA digestion. We describe a gel-less method for DNA fragment isolation after plasmid DNA digestion which is based on in-solution negative selection through ...
PlasmidPURE Miniprep Kit performs well on both high and low copy number plasmids. Typical results show a consistently high quality plasmid DNA in predominantly supercoiled form. The plasmid can be easily cut with restriction enzymes as demonstrated by analysis on agarose gel electrophoresis. ...
A restriction endonuclease is an enzyme that cuts the DNA molecule at, or near to, a specific nucleotide sequence to produce discrete DNA fragments that can be separated by gel electrophoresis. The pattern (‘fingerprint’) of fragments produced is dependent on the distribution of the specific ...
Figure 1. AquaStool purified fecal DNA and RNA. Aliquots (5 μl) of the extracted DNA and RNA were electrophoresized in a native 0.8% agarose gel. DNA ladder. DNA extracted by vortexing. DNA/RNA extracted by sonication (The sheared DNA is ready for next generation sequencing. RNA contamin...
The isolated protein had the ability to complement T4 29 amber mutants. The molecular weight of the purified protein was estimated as 75,000 to 85,000 depending on the composition of SDS-polyacrylamide gel used for the assay. 展开 被引量: 1 年份: 1988 ...
Some illustrations are given showing that immobilization has a stabilizing effect on plasmid stability of recombinant microorganisms. Such systems are promising for the successful continuous cultivation and scale-up of recombinant cells in bioreactors. The confinement of plasmid-bearing cells in polysaccharide...
Modelling the impact of variability of fitness effects on plasmid stability In general, mathematical models of plasmid population biology consider a clonal population in which the plasmid produces a constant reduction in growth rate14,18,19,20,21,22,23. These models usually include the rate of pla...
15. Study on transfering and diffusing of antibiotic resistantplasmidin Shigella spp. 痢疾杆菌耐药性质粒的转移与扩散的研究。 youdao 16. Theplasmidwas obtained and identified by alkali splitting and gel electrophoresis. 碱裂解法抽提质粒,琼脂糖电泳分析目的质粒。
were subjected to fast protein liquid chromatography (FPLC) gel filtration on a Superose 6 (10/300GL) column (GE Pharmacia AKTA explorer) =-=[31]-=... DA Purcellhuynh,RV Farese,DF Johnson,... - 《Journal of Clinical Investigation》 被引量: 410发表: 1995年 High-performance liquid chromat...
a higher resolution than vertical agarosegel electrophoresis, later showed that strain IL106 contains two large plasmids of 102 kb and 115 kb. It was shown that thenapAgene, thenirKgene and thenorCBgenes, encoding the nitrate, nitrite andnitric oxide reductases, respectively, are located on ...