We observed two plasmid types of either plasmid multimers only or monomeric topoisomers only in various conformations (from here on termed pIND1 and pIND2 replicate populations, respectivly; Fig. 3d and Supplementary Fig. 8b). The presence of plasmid multimers is expected to be reflected in an...
Interestingly, DnaK modulates the amyloidogenesis of the RepA-WH1 prionoid in vivo by shifting the equilibrium between an acutely and a mildly cytotoxic transmissible conformations (strains) of the protein towards the latter16. The understanding of plasmid partition23, i.e. the molecular mechanism ...
Analysis of the DNA by gel electrophoresis showed that the DNA was released in open-circular (‘nicked’) and supercoiled conformations, and in vitro cell transfection assays confirmed that the released DNA was transcriptionally active. Arterial injury was induced in the left common, carotid arteries...
Structural insights into the redox-regulated dynamic conformations of human protein disulfide isomerase Antioxid. Redox Signal, 19 (2013), pp. 36-45 Google Scholar 68 P.H. Bessette, J. Qiu, J.C. Bardwell, J.R. Swartz, G. Georgiou Effect of sequences of the active-site dipeptides of Dsb...
conformations also recognized by topoisomerases, but select the major groove of DNA for binding in contrast to topoisomerases that insert into the minor groove, the cIF proteins were able to stimulate the enzymes in their supercoil-relaxing activity on both negatively and positively supercoiled ...
The DNA was quantified using Qubit Fluorometric Quantitation and the quality was checked on an agarose gel. The library was prepared using TruSeq Nano DNA Sample Preparation Kit (Illumina) according to the manufacturer’s instructions and then sequenced on the Illumina MiSeq at Fasteris SA. For ...
Current methods to characterize plasmids include S1-coupled pulsed field gel electrophoresis (PFGE) for plasmid number and sizing6, conjugation-based methods for single plasmid studies and PCR-based or hybridization-based methods for detection of genes and plasmid types7. All these methods suffer from...