[18] developed a PCR-based plasmid typing method based on the replication regions from various plasmid incompatibility groups occurring in Enterobacteriaceae. A similar approach was recently described for the d
Plasmid incompatibility grouping was established by PCR replicon typing using 18 pairs of primers to amplify FIA, FIB, FIC, HI1, HI2, I1-I绾 , L/M, N, P, W, T, A/C, K, B/O, X, Y, F and FIIA replicons. Antibiotic resistance phenotypes were conjugally transferred from S. ...
The above typing schemes were gradually abandoned and successfully replaced by the use of plasmid incompatibility (Novick et al., 1976; Novick and Richmond, 1965) as the basis for plasmid classification (Novick et al., 1976). This was the result of a great consensus effort between research gro...
Thirty-seven plasmid systems contained sequences that could be classified into seven different plasmid incompatibility types (Inc11, Inc18, IncFIB, IncFIC, IncI-ɣ/K1, IncK2/Z and IncW; Supplementary Table 9). Furthermore, 602 plasmid systems contained at least two non-redundant compound ...
Plasmid incompatibility types, antibiotic resistance genes, and insertion elements were predicted using PlasmidFinder 2.1 (95%, minimum threshold for identity; 60%, mini- mum coverage)28, ResFinder (90%, minimum threshold for identity; 60%, minimum coverage)29, and I Sfinder30. Phylogenetic ...
Plasmid incompatibility types were determined using the Plasmidfinder database accessed on December 6, 202398 implemented on ABRicate v.1.0.1 (https://github.com/tseemann/ abricate). To identify instances of genome rearrangement and gene loss, plasmid sequences were aligned, visualized and ...
Incompatibility pMLST: Plasmid multi-locus sequence typing MAC: Mycobacterium avium Complex MCL: Markov Cluster Algorithm NCBI: National Center for Biotechnology Information NTM: Non-tuberculous mycobacteria ORF: Open reading frame RBH: Reciprocal best hits TIR: Terminal inverted repeat VFDB...
In silico detection and typing of plasmids using PlasmidFinder and plasmid multilocus sequence typing. Antimicrob Agents Chemother. 2014;58(7):3895–903. Article PubMed PubMed Central Google Scholar Novick RP. Plasmid incompatibility. Microbiol Rev. 1987;51(4):381–95. Article CAS PubMed Pub...
enterica serovar Stanley reported in China, with similar size and both belonging to plasmid incompatibility group A/C. Conclusion: The present study demonstrated for the first time the co-existence of rmtC and blaNDM-1 in a novel ST192 K. aerogenes. The spread of plasmids harboring both blaNDM...
plasmids by identification of specificrepliconareas extends the method for the identification of plasmid incompatibility groups and also for investigating the spread of plasmids through the food chain. Molecular typing methods based on the characterization of plasmid DNA include plasmid profile typing, ...