Cell-free DNADiffuse large B cell lymphomaAggressive T cell lymphomaNK/T cell lymphomaCell-free DNA (cfDNA) can be released from tumor cells during proliferation and apoptosis; thus, a fraction of the cfDNA in patients with cancer is tumor-derived. However, the prognostic value of cfDNA in ...
ThruPLEX Plasma-seq Kit技术3步反应即可完成针对cell free DNA的建库工作。1~30 ng含量的cf DNA首先高效的进行末端修复。通过采用茎环形接头(无单链尾巴)进行高效平端连接,降低了背景。封闭的5’端阻止了接头-接头二聚体形成。随后未使用的接头被降解,进一步降低背景。高保真度扩增的方式完成以上反应,并获得含有...
Plasma cell-free DNA (cfDNA) are small molecules generated through a non-random fragmentation procedure. Despite commendable translational values in cancer liquid biopsy, however, the biology of cfDNA, especially the principles of cfDNA fragmentation, remains largely elusive. Through orientation-aware anal...
Circulating cell-free DNA (cfDNA) has recently been shown to be a promising prognostic biomarker. The aim of this study was to investigate whether baseline cfDNA measurement could improve the prognostic information of the Royal Marsden Hospital (RMH) score.Solid tumour patients referred for phase I...
Conclusion: Plasma cell-free DNA may be a new promising, independent, non-invasive biomarker for hepatocellular damage.doi:10.4236/aa.2012.24023Zhi YanYing-Li HeQian LiMei-Ling CuiKe WangTian-Yan ChenHong-Li LiuYing-Ren ZhaoScientific ResearchAdvances in Anthropology...
Cell-free DNA (cfDNA) is gaining attention as a novel biomarker for oncologic outcomes. We investigated the clinical significance of cfDNA in hepatocellular carcinoma (HCC) patients treated with radiotherapy (RT). Fifty-five patients with HCC who received RT were recruited from two prospective study...
aCell-free fetal DNA in maternal plasma presents interest as it can possibly serve as a pregnancy associated biomarker. For its quantitation, we investigate the use of fluorescent polymerase chain reaction (PCR) amplification of short tandem repeats (STRs) in pregnancies irrespective of sex. 当它...
(cfDNA) isolation methods with the amount of total cfDNA in copies/μL generated with the QIAamp circulating nucleic acid kit (QIA), PME free-circulating DNA Extraction Kit (PME), Maxwell RSC ccfDNA Plasma Kit (RSC), the EpiQuick Circulating Cell-Free DNA Isolation Kit (EQ), and two ...
et al. Increased plasma DNA integrity in cancer patients. Cancer Res. 63, 3966–3968 (2003). CAS PubMed Google Scholar Gao, Y. J. et al. Increased integrity of circulating cell-free DNA in plasma of patients with acute leukemia. Clin. Chem. Lab. Med. 48, 1651–1656 (2010). CAS ...
Cell-free DNA (cfDNA) in human plasma provides access to molecular information about the pathological processes in the organs or tumors from which it originates. These DNA fragments are derived from fragmented chromatin in dying cells and retain some of the cell-of-origin histone modifications. In...