CRISPR-Cas9, its derived base editors and CRISPR activation systems have greatly aided genome engineering in plants. However, these systems are mostly used separately, leaving their combinational potential largely untapped. Here we develop a versatile CRISPR-Combo platform, based on a single Cas9 prote...
CRISPR/Cas9plant genome editingprime editingprime editing-derived techniquesThe clustered regularly interspaced short palindromic repeats(CRISPR)/CRISPR-associated protein 9(Cas9)system is a fast-growing,genome editing technology that has wide applications in identifying gene functions as well as improving ...
Several CRISPR/Cas immune systems have since been characterised in bacteria and archaea [15] with the type II system, typified by the Cas9 protein, being repurposed as an easily programmed tool for facile genome editing. In its natural state, a spacer-containing crRNA hybridises with a non...
The CRISPR-Cas12a system differs from the more commonly used CRISPR-Cas9. PhD student William de Martines from Wageningen University & Research (WUR) has been conducting research aimed at developing disease-resistant plant varieties through genome editing, with the goal of reducing the use of crop ...
近日,学校生命科学学院张勇教授团队联合园艺园林学院宋洪元教授团队、马里兰大学Yiping Qi教授在Plant Communications上发表了题为Boosting genome editing in plants with single transcript unit surrogate reporter systems的研究论文。该研究工作基于...
Here, we demonstrate CRISPR/Cas9- mediated genome editing in the commercially important coniferous tree P. radiata using both transgenic-mediated production of the editing complex and through the direct delivery of RNPs. CRISPR/Cas is much simpler to implement than the other reported systems such as...
Genome editing in plants using CRISPR type I-D nuclease Article Open access 06 November 2020 Boosting plant genome editing with a versatile CRISPR-Combo system Article 20 May 2022 Expanding the scope of plant genome engineering with Cas12a orthologs and highly multiplexable editing systems Ar...
Rapid Construction of Multiplexed CRISPR-Cas9 Systems for Plant Genome Editing Multiplex CRISPR-Cas9 nuclease mediated genome editing is an efficient method for disrupting gene function in plants. Use of CRISPR-Cas9 has escalated rapi... L Lowder,A Malzahn,Y Qi 被引量: 24发表: 2017年 CRISPR/Cas...
Although numerous efforts have been made, the HDR-mediated precise genome-editing method is still not well established in plants, largely due to extremely limited recombination frequencies and the delivery barrier of high copy number of exogenous donor. CRISPR/Cas9-mediated base-editing systems were ...
Genome-editing technologies using CRISPR–Cas nucleases have revolutionized plant science and hold enormous promise in crop improvement. Conventional transgene-mediated CRISPR–Cas reagent delivery methods may be associated with unanticipated genome changes or damage1,2, with prolonged breeding cycles involving...