Plant systems utilize a diverse array of cytochrome P450 monooxygenases (P450s) in their biosynthetic and detoxification pathways. The classic forms of these enzymes are heme-dependent mixed function oxidases that utilize NADPH or NADH and molecular oxygen to produce functionalized organic products. The...
Zhao X, Liu CJ. Biocatalytic system for comparatively assessing the functional association of monolignol cytochrome P450 monooxygenases with their redox partners. Methods in Enzymology (Academic Press). 2022; 676:133-158. Gou M, Yang X, Zhao Y, Ran X, Song Y, Liu CJ. Cytochromeb5 Is an Obl...
These methods, which are being widely used in mammalian P450 structure-function studies, can allow plant biologists to define critical residues interacting with substrates and, in a directed fashion, alter the reactivities of individual monooxygenases. The homology modelings that have been done on a ...
Molecular-genetic analysis of plant cytochrome P450-dependent monooxygenases. Annu. Rev. Plant Physiol. Plant Mol. Biol. 49, 311-343 Cytochrome P450-dependent monooxygenases are a large group of heme-containing enzymes, most of which catalyze NADPH- and O2-dependent hydroxylation reactio... C ...
The cytochrome P450-dependent monooxygenases are an extremely important metabolic system involved in the metabolism of endogenous compounds and xenobiotics... JG Scott,Z Wen - 《Pest Management Science》 被引量: 514发表: 2001年 Diversification of P450 Genes During Land Plant Evolution Plant cytochromes...
最新研究表明,ABA在拟南芥叶片的快速积累也可以通过促进ABA糖基水解酶(β-glucosidases, AtBG1和AtBG2)水解无活性的糖基化ABA (ABA-glucose ester,ABA-GE)产生有活性的ABA和抑制ABA羟基化酶(cytochrome P450 monooxygenases, CYP707A1-4)对有活性的ABA进行羟基化使其活性降低甚至失活来实现(Lee et al., 2006; ...
Cytochrome P450 monooxygenases (P450s) catalyze a wide variety of monooxygenation reactions in primary and secondary metabolism in plants. The share of P450 genes in each plant genome is estimated to be up to 1%. This implies that the diversification of P450 has made a significant contribution to...
Human drug﹎etabolizing cytochrome P450 monooxygenases (CYPs) feature enormous substrate promiscuity, making them promising tools for the expansion of natural product diversity. Here, we utilized CYP3A4 for the targeted diversification of a plant biosynthetic route leading to monoterpenoid indole alkaloids...
The toxicology of the pyrrolizidine alkaloids is highly dependent on their biotransformation. Hydrolysis and N-oxidation promote detoxification and excretion, hence N-oxide forms are typically less toxic than their parent compound [17]. However, oxidation via cytochrome-P450 monooxygenases can produce dehy...
by hepatic cytochrome P450 monooxygenases (CYPs) (see below). Co-carcinogens are substances that upregulate expression and activity of CYPs and thereby enforce the conversion of pre-carcinogens to activated carcinogens. Physical carcinogenesis Ionizing radiation and UV light cause DNA damage. If damaged...