植物细胞培养(plant cell culture) 植物细胞培养(Plant cell culture) 及次生代谢产物生产 第一节 悬浮培养 一、愈伤组织的诱导 较好的松散性 较强的增殖能力和再生能力 选择适宜的植物外植体材料(幼胚等); 培养基的附加成分对建立疏松易散的愈伤组织具有较大的影响(生长素2,4-D等); 愈伤组织培养阶段必须进行...
植物细胞培养(Plant cell culture)植物细胞培养(Plantcellculture)及次生代谢产物生产 第一节悬浮培养一、愈伤组织的诱导 较好的松散性较强的增殖能力和再生能力 选择适宜的植物外植体材料(幼胚等);选择适宜的植物外植体材料(幼胚等);培养基的附加成分对建立疏松易散的愈伤组织具有较大的影响(的愈伤组织具有较大的影响...
Plant Tissue Culture Media What’s really important? Plant Tissue Culture Why does it work? Plant cells – Dedifferentiate Plant cell division- Somatic cells are diploid Mitosis – Chromosomes duplicate and divide Meiosis – The process of forming sex cells, 2n splits and become 1n gametes Major...
culturepptplanttissuecytokinincallusauxin -1 -2 -3 thecultureofplantseeds,organs, tissues,cells,orprotoplastson nutrientmediaundersterile conditions. -4 BasisforPlantTissueCulture •TwoHormonesAffectPlantDifferentiation: –Auxin:StimulatesRootDevelopment –Cytokinin:StimulatesShootDevelopment •Generally,theratio...
Organ culture 1. shoot tip culture 2. root culture 3. leaf culture 4. anther culture ? Callus culture ? Cell suspension culture ? Protoplast culture Tissue Culture Applications ?Micropropagation ?Germplasm preservation ?Somaclonal variation ?dihaploid production ?Protoplast fusion ?Secondary metabolites ...
Plant regeneration through culture of isolated microspores of Triticum aestivum L. Wheat microspores mechanically isolated from the anthers before culture and isolated from the anthers during the hole culture period in a chemically define... IKD Tuvesson,RCV ?Hlund - 《Plant Cell Tissue & Organ...
–Inmanycases,differentgenotypeswithinaspecieswillhavevariableresponsestotissueculture;responsetosomaticembryogenesishasbeentransferredbetweenmeloncultivarsthroughsexualhybridization 7 ThreeFundamentalAbilitiesofPlants ✓Totipotency thepotentialorinherentcapacityofaplantcelltodevelopintoanentireplantifsuitablystimulated.It...
4、or separation and purification)醫葯品等有用物質生產醫葯品等有用物質生產Production for medicaments 細胞培養細胞培養(Cell culture)植物無性繁殖植物無性繁殖(Asexal propagation)細胞固定細胞固定(Cell fixation)種源保存種源保存(Germplasm preservation)遺傳修飾遺傳修飾(Genetic modification)必要技術應用技術 Q :...
Initial culture of freshly isolated microspores i... Miyoshi,Kazumitsu - 《Plant Cell Reports》 被引量: 160发表: 1996年 Increased α-tocopherol content in soybean seed overexpressing the Perilla frutescens γ-tocopherol methyltransferase gene One transgenic plant was recovered and the progeny was ...
(Fig.4b). Infection withM. acerinacaused cell integrity loss, severe plasmolysis, and organelle disintegration of various degrees in the PNLs. Severe chloroplast degradation was observed, including disruption of the chloroplast membrane and degradation of grana and stroma thylakoids (Fig.4b), ...