22. Over the course of this study, inter- and intra-species responses were observed for peak cell densities, number of population doublings, passaging times and culture lifespan. Cells remained in stationary phase for up to 1 week, depending on the species and the culture...
Cell culture treatments In cell–cell interaction experiments, epicardioids were treated with 0.25 µM, 0.5 µM or 1 µM linsitinib (Tocris, 7652) or 200 µg ml–1 or 500 µg ml–1 NRP2 blocking antibody (R&D, AF2215) in maintenance medium on days 11, ...
6-MSAS also served as a model enzyme to investigate the influence of bacterial (Sfp) and fungal (NpgA) PPTases on polyketide biosynthesis inS. cerevisiae[80]. This study revealed that the fungal PPTase NpgA outperforms Sfp in respect of 6-MSA product titers. The achieved titer was even s...
250 μM sodium butyrate (Sigma,303,410-100G), 0.3 μM cyclic pifithrin-a (Stemgent,04–0040) were added to the RM culture medium to facilitate the transition from UCs to UiPSCs. Subsequently, in the later stage (day 11 to day 17), RM was replaced with mTeSR1 medium (stemcell,85,8...
These results suggest that the site of Tn insertion affected the adhesion property in different culture media, although the underlying mechanism remains unclear. None of the non-adhesive mutants could grow on mucin (Figure 2B), indicating that the housekeeping sortase is responsible for anchoring and...
Cell-block sections of cell lines: (a) Gross photo of glass slides with cell-block sections stained with hematoxylin and eosin (HE) on the left with IHC for cytokeratin (CK) 7 on the right. (b) Pancreatic adenocarcinoma cell line (HE stained). (c) Pancreatic adenocarcinoma cell line (im...
Cells (2 × 106 cells/mL) were starved for 12 h in their respective basic culture media containing 0.5 % BSA and stimulated afterwards for 5 min with 1,25-dihydroxyvitamin D3 at various concentrations (10−10–10−8 M) or with vehicle (DMSO) only. The experiment was carried out...
In this review, we describe the generation and use of cell culture models of transmissible spongiform encephalopathies, also known as prion diseases. These... F Béranger,A Mangé,J Solassol,... - 《C R Biol》 被引量: 75发表: 2001年 Chemoselectively addressable template: a valuable tool ...
Culture conditions For GS115-NpgA-HIS6, a singal colony was inoculated into a 250 mL shake flask containing 25 mL YPD medium and cultivated at 30°C and 200 rpm for about 16 hours. Afterwards, the broth was transferred into 25 mL MGY medium to make the OD600about 0.03. The liquid was...
3D Cell Culture on VitroGel SystemFigure 5. OP9 GFP (top) and U87MG (bottom) cells in different VitroGel Hydrogels. The results show V-RGD-PLUS and V-LDP2 support OP9 fibroblast morphology and U87 epithelial morphology, however, V-3D supports cell growth but not the characteristic ...