22. Over the course of this study, inter- and intra-species responses were observed for peak cell densities, number of population doublings, passaging times and culture lifespan. Cells remained in stationary phase for up to 1 week, depending on the species and the culture...
Cell culture treatments In cell–cell interaction experiments, epicardioids were treated with 0.25 µM, 0.5 µM or 1 µM linsitinib (Tocris, 7652) or 200 µg ml–1 or 500 µg ml–1 NRP2 blocking antibody (R&D, AF2215) in maintenance medium on days 11, ...
Diminished Follicle Reserve in Aged Ovaries from Cynomolgus Monkeys We obtained ovaries from four juvenile (4–5 years old) and four aged (18–20 years old) cynomolgus monkeys (Appt et al., 2010,Drevon-Gaillot et al., 2006) (Figure S1A). As species of the genusMacacaundergo menopause a...
6-MSAS also served as a model enzyme to investigate the influence of bacterial (Sfp) and fungal (NpgA) PPTases on polyketide biosynthesis inS. cerevisiae[80]. This study revealed that the fungal PPTase NpgA outperforms Sfp in respect of 6-MSA product titers. The achieved titer was even s...
Fetal human cortical neurons grown in culture: morphological differentiation, biochemical correlates and development of electrical activity. Int J Dev Neurosci. 1999;17(4):347–56. Article PubMed Google Scholar Cummins TR, Xia Y, Haddad GG. Functional properties of rat and human neocortical ...
Cells (2 × 106 cells/mL) were starved for 12 h in their respective basic culture media containing 0.5 % BSA and stimulated afterwards for 5 min with 1,25-dihydroxyvitamin D3 at various concentrations (10−10–10−8 M) or with vehicle (DMSO) only. The experiment was carried out...
medically ill Manual MN scoring enabled 48-hour culture triage dose estimations in 8 minutes for unexposed donors, while donors exposed to 2 or 4 Gray needed 20 minutes. For high-dose scoring, one hundred BNCs can be utilized effectively, eliminating the need for two hundred BNCs in triage...
In this review, we describe the generation and use of cell culture models of transmissible spongiform encephalopathies, also known as prion diseases. These... F Béranger,A Mangé,J Solassol,... - 《C R Biol》 被引量: 75发表: 2001年 Chemoselectively addressable template: a valuable tool ...
Culture conditions For GS115-NpgA-HIS6, a singal colony was inoculated into a 250 mL shake flask containing 25 mL YPD medium and cultivated at 30°C and 200 rpm for about 16 hours. Afterwards, the broth was transferred into 25 mL MGY medium to make the OD600about 0.03. The liquid was...
2005b. Evaluation of unpurified cell culture supernatant as template for the polymerase chain reaction (PCR) with largemouth bass virus. Journal of Aquatic Animal Health 17:191-196.J. M. Grizzle, and J. E. Schneider, Jr. 2005b. Evaluation of unpuri- fied cell culture supernatant as ...