目的:观察和评估"疏肝调神"法针刺对PTSD模型大鼠焦虑,恐惧情绪和终纹床核神经元形态损伤的改善作用,并检测和分析针刺对PKC/ERK/CREB信号通路关键蛋白表达的影响,以揭示和探讨"疏肝调神"法针刺干预PTSD神经损伤的分子生物学机制. 方法:从50只雄性SD大鼠中随机选出10只作为空白组.剩余40只大鼠采用幽闭电击法联合负重力...
PKC_ERK_CREB通路在ERα介导围生期BPA暴露对子代雄性小鼠神经行为功能损伤的机制探讨 热度: PKCg激活机制及其功能术 许顺江 丛 斌(河北医科大学病理生理学教研室,石家庄050017) 摘要 蛋白激酶C∈(proteinkinaseC∈,PKC∈)是非典型PKC家族成员,广泛参与细胞功能的调节。
Total PKCζ/λ and Bad (a) or total PKCδ and ERK (b) were used as loading control. (c) Phosphorylation of CREB and ATF-1 is downstream of PKC activation in response to IR. Western blot analysis of irradiated MRC-5 cells (4 h recovery) that were treated either with the PKC-...
Mutations on the AP-1 or the GRE half sites of cGRE showed that GR binds as an heterodimer on cGRE, and PKC or MEK inhibitors diminish binding at the AP-1 site. PKC and ERK signaling thus modulate GR activity and its interaction with CREB or AP-1 at the TRH gene promoter. 展开 ...
第三部分 SSTA疗效与PKCδ相关信号通路的相互作用及其与gsp癌基因的关系 目的探究SSTA疗效与PKCδ相关信号通路因子的关系,及其与gsp癌基因的联系 方法使用SSTA干预原代培养的人垂体GH腺瘤细胞,GH-Elisa检测各实验分组细胞分泌GH水平变化,CCK-8检测各实验分组细胞活性变化,Western blot检测PKCδ,CREB,ERK1/2蛋白磷酸化...
Subsequently, the results of the luciferase assay using internal deletion clones showed that the EBS and CREB/ATF binding sites in the EGR-1 promoter are required for TA-induced EGR-1 transactivation. Experiments using kinase inhibitors and siRNA transfection implied the involvement of the PKC/ERK ...
Thermo PV3508 FLUORESCEIN-CREBTIDE SUBSTRATE 1 MG ￥11,027.00 1 MG Thermo PV3509 FLUORESCEIN-CROSSTIDE SUBST. 1 MG ￥11,027.00 1 MG Thermo PV3610 FLUORESCEIN-POLY GT 1 ML ￥11,027.00 1 ML Thermo PV4445 GFP-ATF2 (19-96) 20 NMOL ￥11,027.00 20 NMOL Thermo PV4446 GFP-C-JUN (1-179...
We finally showed using SB203580 and RO-31-8220 that phosphorylation of CREB and CREB-promoter activity induced by FA are under the control of both p38 MAPK and PKC. Taken together, the results showed that FA uses different pathways to induce IL-11 expression in lung BEAS-2B cells. IL-11...
Although the EGF-activated PI3K/Akt/CREB signaling axis upregulates SGLT1 expression and enhances glucose uptake in intestine epithelial cells [62], the tyrosine kinase activity of EGFR or IGF1R and Akt signals are not needed for SGLT1 upregulation in cancer cells [46, 63]. In this study, ...
PI3K/Akt and CREB regulate adult neural hippocampal progenitor proliferation and differentiation. Dev Neurobiol. 2007;67:1348–61. Article CAS PubMed Google Scholar Yang F, Gao JY, Chen H, Du ZH ZXQ, Gao W. Targeted inhibition of the phosphoinositide 3-kinase impairs cell proliferation, ...