pKa
The fluorescence lifetime of Dbo in the peptides ( τ ) was measured as a function of pH. The side chains collide with Dbo intramolecularly and quench it efficiently only when they are deprotonated (i.e., pH≥side chain p K a ). The p K a values of the H 6 , K 6 , and R ...
Accuracy of Density Functionals in the Prediction of Electronic Proton Affinities of Amino Acid Side Chains The ionization states of amino acids influence the structure, function, stability, solubility, and reactivity of proteins and are difficult to determine un... NF Brás,MAS Perez,PA Fernandes,...
As applications, we have derived the p K a value of the side chain of an amino acid and almost reproduced the experimental value. By using our computing schemes, we showed the influence of hydrogen bonds on the p K a values in the case of tripeptides, which decreases the p K a value...
Here we show that the uniform influence of the solvent on chemical shifts of exposed lysine methyl groups could be overcome by adjusting the pH of the buffering solution closer to the pKa of lysine side chains. Under these conditions, due to variable pKa values of individual lysine side chains...
Thus, the tethering region of the organelle-inserted anchor protein is properly oriented and avidly binds RII (PKAII) isoforms in intact cells. Two critical domains in S-AKAP84 were mapped. Residues 1 to 30 govern insertion of the polypeptide into the outer mitochondrial membrane; amino acids ...
01 July 2016 Polysaccharide Nanoparticles for Efficient siRNA Targeting in Cancer Cells by Supramolecular pKa Shift Ying-Ming Zhang1,Yang Yang1,†,Yu-Hui Zhang1 &Yu Liu1,2 Biomacromolecular pKa shifting is considered as one of the most ubiquitous processes in biochemical events, e.g....
The simulated mAb-1 structure indicated the microenvironment of HC-CDR3-Lys98 is less polar than HC∗-CDR3-Lys98, not only due to the surrounding amino acids but also due to the orientation of their side chains. Within 4.5 Å, HC-CDR3-Lys98 is surrounded by aromatic rings from ...
This is the largest pKa shift for an A residue in structured nucleic acids reported so far, and similar in size to the largest pKa shifts observed for amino acid side chains in proteins. Both RNA pre-folding and ligand binding contribute to the pKa shift.Wiley...
It was also possible to determined the p K a values some amino acids containing ionizable side-chains. This study shows that, in order to understand fully the retention behaviour of peptides containing an α-amino group in reversed-phase chromatography, one must incorporate an α-amino group ...