磷脂酰肌醇3-激酶(PI3Ks)蛋白家族参与细胞存活、生长、代谢和血糖稳态等多种细胞功能的调控。 PI3K激酶活性升高通常与多种癌症相关。顾名思义,PI3Ks磷酸化磷脂酰肌醇PI(一种膜磷脂)肌醇环的第3位碳原子。PI在细胞膜组分中所占比例较小,比磷脂酰胆碱、磷脂酰乙醇胺和磷脂酰丝氨酸含量少, 但在脑细胞膜中,含量较...
Here we showed that expression ofPIK3CA-activating mutations in human EC induced hyperproliferation and concomitant cellular senescence.PIK3CA-induced senescent cells are larger than normal EC and are characterized by many intracellular vesicles and elevated β-galactosidase activity. Oncogene-induced senescenc...
Plus, ADORA2A was relatively enriched in the biological functions of the cAMP signaling pathway and negatively regulated cysteine-type endopeptidase activity involved in apoptotic processes (Fig. 1B). Subsequently, we verified ADORA2A expression in colorectal cancer cell lines with stable SETDB1 ...
The cell survival rates of the different groups were measured with the 34,5ヾimethylthiazoll)3ヽarboxymethoxyphenyl)4﹕ulfophenyl)‐tetrazolium assay kit; the lactate dehydrogenase (LDH), malondialdehyde (MDA) content, and superoxidedimutase (SOD) activity in the bathing medium were assayed for ...
Cytolytic activity TAMs: Tumor-associated macrophages LAG‐3: Lymphocyte activation gene‐3 CTLA‐4: Cytotoxic T-lymphocyte-associated protein 4 ELISA: Immunosorbent assay ICI: Immune checkpoint inhibitor CAR-T: Chimeric antigen receptor T-cell ISGs: Interferon-stimulated genes IRFs: IFN ...
Luciferase assay was performed 36 h after transfection. Results are represented as relative luciferase activity. D, the effect of miR-26a on the RCN2 mRNA levels in GEECs. E and F, miR-26a down-regulated RCN2 protein in GEECs. G and H, the RCN2 mRNA levels in GEECs transfected with...
内质网应激通过miRNA-155作用PI3K-Akt-mTOR信号通路促进狼疮性肾炎进展的研究 内质网应激通过m i R N A Ｇ１５５作用P I ３K ＧA k t Ｇm T O R 信号通路促进狼疮性肾炎进展的研究 杨㊀明１,房叶华２(中南大学湘雅医学院附属株洲医院,１．肾内科,２．临床心理科,湖南株洲４１２０００)D O I :１０．...
We observed that MTOR, which encodes mTOR, was the top gene that, when knocked down, re-sensitized resistant cells to the antiproliferative activity of BYL719 (Figure 1E; Figure S1E; Table S1). Taken together, these results show that SCC cells with acquired resistance to BYL719 maintain ...
HEK293T or PC3 cells were harvested using non-denaturing lysis buffer (as per the Smad2 ubiquitination assay) to retain kinase activity and supplemented with phosphatase and protease inhibitors as per immunoblotting. Active Akt was immunoprecipitated using anti-pAkt (S473) antibody and was tested ...
(MELAS). We have found that the basal expression and activity of the PI3K-Akt-mTORC1 pathway were increased in the mutant cells, and were strongly associated with redox imbalance, oxidative stress, and glucose dependence. Phosphorylation of Akt and ribosomal protein S6 (a downstream target of ...