Department of Veterinary Medicine, University of Cambridge, Madingley Road, Cambridge, UK Clare E. Bryant Corresponding author Correspondence to E. Liana Falcone. Additional information Competing interests The authors declare that they have no competing interests. Authors’ contributions ELF, CEB, and SMH...
This was washed 3 times in water and further purified by re-crystallization. Protein size and purity was assessed by staining with Coomassie Brilliant Blue (BDH Laboratory Supplies, Poole, UK) after SDS-PAGE (16), and the yield was determined by the Bio-Rad protein assay (Bio-Rad) (17)....
(Tecan GmbH., Groding, Austria), UV/Visible spectrophotometer (Ultrospec 6300 Pro. Bichrom Ltd. UK), luminometer (Turner Biosystems, 23 CA, USA), Centrifuge SIGMA 3K15 (SIGMA Laborzentrifugen, Osterode am Harz, Germany) and Centrifugal concentrators (NANOSEP™, Pl Filtration, USA) were ...