All drugs were given in the drinking water. Systolic blood pressure (SBP) was measured by the tail-cuff method. RNA Preparation and Real-Time Quantitative Polymerase Chain Reaction At 32 weeks of age, the rats were anesthetized with ether and killed by decapitation. The aortas were quickly ...
Mice were administered filter-sterilized 3.5 % (w/v) DSS (m.w. 36,000–50,000; MP Biomedicals)-supplemented drinking water ad libitum for 7 days, followed by 1 day of regular autoclaved water. Mice were monitored daily for weight, disease activity, and survival. The DAI was scored as ...