First, we identified the two kinases which phosphorylate the linker domain of Drosophila Mad using dsRNA in Drosophila S2 cells; we show that phosphor- ylation of serine 212 was carried out by Cdk8 which then acts as the priming phosphate to allow the subsequent second and third phos- phory...
T-loop phosphorylation is carried out by a trimeric CDK-activating kinase (CAK, a complex of CDK7, cyclin H and MAT1) and opposed by CDK-associated phosphatase (KAP) and protein phosphatase 2C-like proteins (Fesquet et al., 1993; Poon et al., 1993; Solomon et al., 1993; Fisher ...
Anoxygenic photophosphorylation is carried out by purple bacteria, green bacteria, and others (Bryant & Frigaard, 2006), and can involve diverse electron donors other than water. The waste products are thus similarly diverse but are never O2. All forms of photophosphorylation use light ene...
intracellular ATP level to generate a status that is highly addictive to OXPHOS. Furthermore, the combinational inhibition of mitotic kinase and OXPHOS by alisertib, and metformin respectively, generates severe energy exhaustion in mitotic cells that consequently triggers cell death. The combination regim...
Transphosphorylation to methanol did not take place when the catalytic hydrolysis was carried out by Ce^ in a weakly acidic medium, where the liberation of salicylic acid is maximum, perhaps because the active phosphate produced immediately combines with Ce^ to be excluded from the reaction system...
It is argued that this mechanism, based on at least 15 years of experimental and theoretical work by Sunil Nath, constitutes a fundamentally different theory of the energy conversion process that eliminates all the inconsistencies in Mitchell's chemiosmotic theory pointed out by other authors. It ...
Through phosphorylation and dephosphorylation of Ser-3, the activity of CFL-1 is specifically regulated by protein kinases such as LIM-kinases (LIMKs) and testicular protein kinases (TESKs), as well as phosphatases such as slingshot (SSH) family members and chronophin[8,9]. The regulation of...
andgene transcription.Phosphorylation and dephosphorylationare carried out by kinases andphosphatases, respectively. There are currently predicted to be 518 kinases and ~125 phosphatases encoded in thehuman genome, further underscoring the overall importance of phosphorylation in molecular regulation.Phosphatases...
Phosphorylation modification is usually carried out by dry heating or water bath heating. In previous studies (Li, Zhuo, Ma, Jin, & Long, 2018), it was shown that microwave-assisted phosphorylation could dramatically accelerate the reaction extremely and that phosphorylation of egg white protein ...
Although Jhd2 distributed throughout cells, the strong Jhd2 phosphorylation signals were observed in the cytoplasm (Fig. 2m), indicating that the phosphorylated Jhd2 is primarily localized in the cytoplasm. Jhd2 has a bipartite nucleus localization signal (NLS) within the sequence 313RLLPARKLSIDELE...