Generally speaking, precise regulation of protein phosphorylation is carried out by the opposing activities of protein kinases (enzymes that transfer phosphate groups to tyrosine or serine/threonine residues on protein substrates) and protein phosphatases (enzymes that remove phosphate groups from ...
8d). Altogether, the results are consistent with the hypothesis that the phosphorylation is carried out by Cdk1. To corroborate this, Cdk1 immunocomplexes prepared from TKO and TKO-Myc MEFs were subjected to in vitro kinase assays using His-p27 as substrate. The results showed that ...
T-loop phosphorylation is carried out by a trimeric CDK-activating kinase (CAK, a complex of CDK7, cyclin H and MAT1) and opposed by CDK-associated phosphatase (KAP) and protein phosphatase 2C-like proteins (Fesquet et al., 1993; Poon et al., 1993; Solomon et al., 1993; Fisher ...
intracellular ATP level to generate a status that is highly addictive to OXPHOS. Furthermore, the combinational inhibition of mitotic kinase and OXPHOS by alisertib, and metformin respectively, generates severe energy exhaustion in mitotic cells that consequently triggers cell death. The combination regim...
It is argued that this mechanism, based on at least 15 years of experimental and theoretical work by Sunil Nath, constitutes a fundamentally different theory of the energy conversion process that eliminates all the inconsistencies in Mitchell's chemiosmotic theory pointed out by other authors. It ...
The aim of the current research is to identify genomic SNPs associated with starch phosphorylation by carrying out a genome-wide association study in potatoes. Results A total of 90 S. tuberosum L. varieties were used for phenotyping and genotyping. The phosphorus content of starch in 90 ...
Transphosphorylation to methanol did not take place when the catalytic hydrolysis was carried out by Ce^ in a weakly acidic medium, where the liberation of salicylic acid is maximum, perhaps because the active phosphate produced immediately combines with Ce^ to be excluded from the reaction system...
Through phosphorylation and dephosphorylation of Ser-3, the activity of CFL-1 is specifically regulated by protein kinases such as LIM-kinases (LIMKs) and testicular protein kinases (TESKs), as well as phosphatases such as slingshot (SSH) family members and chronophin[8,9]. The regulation of...
Additional experiments of in vitro phosphorylation of OPN and BSP by several other known protein kinases were carried out. cAMP-dependent protein kinase showed no phosphorylation of OPN or BSP, while protein kinase C and cGMP-dependent protein kinase led to minor phosphorylation, each of the latter...
Phosphorylation modification is usually carried out by dry heating or water bath heating. In previous studies (Li, Zhuo, Ma, Jin, & Long, 2018), it was shown that microwave-assisted phosphorylation could dramatically accelerate the reaction extremely and that phosphorylation of egg white protein ...