The phosphorylated epitopes were generated by casein kinase-1 and -2, and phosphorylation led to increased oligomerization and fibrillization of TDP-43. Interpretation These results suggest that phosphorylated TDP-43 is a major component of the inclusions, and that abnormal phosphorylation of TDP-43 ...
–streptavidin conjugates were added. The remaining cells were subsequently incubated with a pool of dual labeled peptide tetramers. The background and experimental tubes were incubated for 60 min at 4 °C with gentle mixing, washed and resuspended at 20 million cells per ml in TBS buffer. Prio...
et al. Downregulation of glutamic acid decarboxylase in Drosophila TDP-43-null brains provokes paralysis by affecting the organization of the neuromuscular synapses. Sci. Rep.8(1), 2018. https://doi.org/10.1038/s41598-018-19802-3 (1809). Article CAS Google Scholar Dolan, P. J. & Johnson...
Development of tau-based therapies for Alzheimer’s disease requires an understanding of the timing of disease-related changes in tau. We quantified the phosphorylation state at multiple sites of the tau protein in cerebrospinal fluid markers across four
The ALS disease protein TDP-43 is actively transported in motor neuron axons and regulates axon outgrowth and suggest that impairment in the post-transcriptional regulation of mRNAs in the cytoplasm of motor neurons may be a major factor in the development of... F Claudia,GJ Bassell,R Wilfried...
(tdp(ep=l =0o.f01.8h1)u8(m)s(easeenedNedtSeatCialLsilCsin,inFtriFegiaugtrumere3n)3.t).with NUC050 and NUC052 significantly improved mouse survival (p = 0.017 and p = 0.033, respectively), while treatment with gemcitabine at 120/80 mg/kg did not (p = 0.18) (see details in ...
1H NMR (500 MHz, CDCl3): δ 7.87–7.74 (m, 4H), 7.57–7.52 (m, 2H), 7.48 (dt, J = 7.2, 3.3 Hz, 4H), 4.57 (td, J = 10.9, 4.4 Hz, 1H), 3.49 (dd, J = 14.7, 1.4 Hz, 2H), 1.71–1.63 (m, 1H), 1.59 (ddd, J = 13.2, 7.1, 3.4 Hz, 1H), 1.34 (tdp, J = ...
P1-140: Aggregation of phosphorylated and ubiquitinated TDP-43 in SH-SY5Y cells 2008, Alzheimer's & Dementia more Takashi Nonaka, Tetsuaki Arai, Haruhiko Akiyama, Emanuele Buratti, Francisco E. Baralle, Masato Hasegawa P1-140: Aggregation of phosphorylated and ubiquitinated TDP-43 in SH-SY5Y cel...
Hasegawa M, Arai T, Nonaka T et al (2008) Phosphorylated TDP-43 in frontotemporal lobar degeneration and amyotrophic lateral sclerosis. Ann Neurol 64:60–70. : 10.1002/ana.21425Hasegawa M, Arai T, Nonaka T, Kametani F, Yoshida M, Hashizume Y, Beach TG, Buratti E, Baralle...
Background: Phosphorylated and proteolytically cleaved TDP-43 is a major component of the ubiquitin positive inclusions in the most common pathological subtype of frontotemporal lobar degeneration (FTLD-U) and amyotrophic lateral sclerosis (ALS). Intracellular accumulation of TDP-43 has been observed in...