Despite its critical role in glucose flux, the biologically relevant crystal structure of the mammalian PFK1 tetramer has not been determined. We report here the first structures of the mammalian PFK1 tetramer, for the human platelet isoform (PFKP), in complex with ATP-Mg2+and ADP at 3.1 and...
production. TRIM21 is upregulated at the site of autoimmune inflammation and may play an important role in the pathogenesis of autoimmunity18. Of note, TRIM21 expression is downregulated in hepatocellular carcinoma cells and is significantly and inversely correlated with patient prognosis, suggesting tha...
The invention relates to a process for the preparation of L-amino acids. The process includes fermenting the coryneform bacteria producing the desired L-amino acid, in which at least the gene coding for 6-phosphofructokinase and/or the gene coding for 1-phosphofructokinase are/is attenuated, enric...
In the longer transcript, an insertion of 252 nucleotides totally homologous to the structure of the 10th intron of the PFKM gene was found separating exon 10 from exon 11. In addition, two single base transitions were identified by direct sequencing: [exon 6; codon 95; CGA (Arg) to TGA ...
Despite its critical role in glucose flux, the biologically relevant crystal structure of the mammalian PFK1 tetramer has not been determined. Here we report the first structures of the mammalian PFK1 tetramer, for the human platelet isoform (PFKP), in complex with ATP-Mg(2+) and ADP at ...
structurepredictionHuman phosphofructokinase-1 (PFK1) has a high sequence similarity to PFK1 proteins belonging to other vertebrates. It seems to have a quite ordered spatial structure presenting a few globular domains. The homologues structural model that can be used for human PFK1 is those of ...
Polyacrylamide gel electrophoresis in presence of sodium dodecyl sulphate and MALDI-TOF spectra showed that the enzyme is composed of subunits of identical size of 100±5 kDa, forming an octameric structure. The N-terminus of the enzyme was found to be blocked. Sequences of tryptic and chy...
Persistence of the catalytic and allosteric properties over the temperature range 20–80 °C suggests that the same protein structure is retained throughout this temperature range. Thermus X-1 phosphofructokinase is more stable to inactivation by heat, urea, guanidine hydrochloride or acidification than...
The frequency of the different amino acids at the first 30 positions starting from the N-terminal end of the PFK structure was also computed in course of this analysis. Relative amino acid usage frequency profile of PFK sequences from Dickeya, Erwinia and Pectobacterium were found to be quite ...
PhosphofructokinaseMycobacterium marinumCrystal structureSMALL-ANGLE SCATTERINGRIBOKINASEEVOLUTIONSITECOLIJi, ChaonengGan, JianhuaLi, JixiGao, BaocaiJi, RuiLi, ZhengyangSu, XiaoqinLi, HongyongSun, YichengBiochemical and Biophysical Research Communications